| HPLC-ELSD测定积雪草提取物中积雪草苷的含量 |
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| 引用本文： | 于泉林,高文远,陈海霞,段宏泉.HPLC-ELSD测定积雪草提取物中积雪草苷的含量[J].中国中药杂志,2007,32(6):503-505. |
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| 作者姓名： | 于泉林 高文远 陈海霞 段宏泉 |
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| 作者单位： | 1. 天津大学,药物与科学技术学院,天津,300072
2. 天津医科大学,药学院,天津,300070 |
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| 摘 要： | 目的：用HPLC-ELSD测定积雪草提取中积雪草苷的含量。方法：色谱柱为HiQ siL KYA-C18分析柱,流动相为乙腈-水,梯度洗脱；漂移管温度105 ℃；载气流速2.9 L·min-1。结果：积雪草苷在0.35～7.0 μg呈良好线性关系；积雪草提取物中积雪草苷的回收率为94.9％(RSD 1.7%)。结论：该方法简便、准确、分离效果好,无干扰,可用于积雪草提取物中积雪草苷的质量评价。
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| 关 键 词： | 积雪草提取物 积雪草苷 质量控制 |
| 文章编号： | 1001-5302（2007）06-0503-03 |
| 收稿时间： | 2006-07-12 |
| 修稿时间： | 2006-07-12 |
Determination of aisaticoside in Centella asiatica extract by HPLC-ELSD |
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| YU Quan-lin; GAO Wen-yuan; CHEN Hai-xia; DUAN Hong-quan.Determination of aisaticoside in Centella asiatica extract by HPLC-ELSD[J].China Journal of Chinese Materia Medica,2007,32(6):503-505. |
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| Authors: | YU Quan-lin; GAO Wen-yuan; CHEN Hai-xia; DUAN Hong-quan |
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| Institution: | College of Pharmaceutical Sciences and Biotechnology, Tianjin University, Tianjin 300072, China. |
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| Abstract: | OBJECTIVE: A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract. METHOD: The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1). RESULT: The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%). CONCLUSION: The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract. |
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| Keywords: | HPLC-ELSD |
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