| Western-Blotting在粘放菌5519 I型菌毛鉴定中的应用 |
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| 引用本文： | 梁景平,刘正,朱敏,黄冬生. Western-Blotting在粘放菌5519 I型菌毛鉴定中的应用[J]. 实用口腔医学杂志, 1999, 15(5): 323-325 |
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| 作者姓名： | 梁景平 刘正 朱敏 黄冬生 |
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| 作者单位： | 上海第二医科大学口腔医学院,200011 |
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| 摘 要： | 目的：检测粘放菌５５１９ Ｉ型菌毛的纯度及 Ｗｅｓｔｅｒｎ Ｂｌｏｔｔｉｎｇ 方法的特异性。方法：采用 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 电泳和 Ｗｅｓｔｅｒｎ Ｂｌｏｔｔｉｎｇ 免疫印迹法。结果： 粘放菌 ５５１９ Ｉ型菌 Ｍｒ 大部分为５４ ０００,少部分为３８ ０００。 Ｗｅｓｔｅｒｎ Ｂｌｏｔｔｉｎｇ 检测结果与 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ结果相一致。结论： Ｗｅｓｔｅｒｎ Ｂｌｏｔｔｉｎｇ 在粘放菌菌毛鉴定中具有高度特异性。
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| 关 键 词： | 菌毛 SDS-PAGE电泳 粘放菌 |
Identification of type I fimbriae in A.viscosus 5519 with Western-Blotting |
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| Liang Jingping,Liu Zheng,Zhu Min,et al.. Identification of type I fimbriae in A.viscosus 5519 with Western-Blotting[J]. Journal of Practical Stomatology, 1999, 15(5): 323-325 |
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| Authors: | Liang Jingping Liu Zheng Zhu Min et al. |
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| Affiliation: | Liang Jingping,Liu Zheng,Zhu Min,et al. College of Stomatology,Shanghai Second Medical University,Shanghai 200011 |
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| Abstract: | bjective:To study the feasibility of Western Blotting in the identification of type I fimbriae in A.Viscosus 5519.Method:SDS PAGE electrophoresis and Western Blotting were carried out to identificate type I fimbriae in A.Viscosus 5519.Results:The molecules of type I fimbriae of A.Viscosus 5519 detected by Western Blotting were consistent with that by SDS PAGE.Conclusion:Western Blotting is feasible in identification of fimbriae of A.Viscosus . |
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| Keywords: | Fimbriae SDS PAGE electrophoresis Actinomyces Viscosus |
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