PRLr mRNA在人体免疫系统中的表达

目的：探讨催乳素受体（PRLr）在人体免疫系统中的表达。方法：临床获取人中枢免疫器官胸腺瘤、骨髓和外周免疫器官淋巴结、外周血单个核细胞，经RNA抽提，RT-PCR扩增PRLr mRNA的特异片段，并进行测序。结果：从胸腺瘤、骨髓、淋巴结及外周血单个核细胞均扩增出PRLr mRNA，其片段长度与预计长度一致，均为276bp，经测序证实为所需片段。结论：人中枢免疫器官胸腺瘤、骨髓以及外周免疫器官淋巴结、外周血单个核细胞存在PRLr表达，从受体角度直接证实内分泌激素PRL发挥免疫调节作用的生物学结构依据。

Expression of PRLr mRNA in human immune system

Objective:To investigate the expression of prolactin receptor(PRLr) in human immune system.Methods:Specimens of human immune system were obtained from both central and peripheral immune organs, including thymus(thymoma), bone marrow, lymph node and peripheral blood mononuclear cells. Specific fragments of PRLr mRNA were obtained through RNA isolation and RT-PCR amplification and confirmed by DNA sequencing.Results:PRLr mRNA was detected from the thymoma, bone marrow, lymph node and peripheral blood mononuclear cells. All the PRLr cDNA fragments generated by PCR were as long as the expected length, which was 276 bp. The sequencing result of the cDNA was identical to the sequence of PRLr cDNA in GeneBank.Conclusion:The study confirmed that prolactin receptor mRNA was expressed in human central immune organs such as thymus and bone marrow and peripheral immune organs such as lymph node and peripheral blood mononuclear cells. This phenomenon provided a biologic structural evidence that neuro-endocrine hormone PRL might play a modulating role on immune cells from receptor's point of view.