病毒载体介导的视网膜基因转移研究进展

用病毒作为载体携带功能基因转染视网膜以达治疗目的，这种基因治疗方法为某些遗传性视网膜病如视网膜色素变性的治疗开辟了新途径。应用腺病毒或腺相关病毒载体，经视网膜下间隙或玻璃体注射，可使报告基因在视网膜细胞中有效表达。腺病毒介导的基因表达起效早，持续时间短，腺相关病毒介导的基因表达起效晚，持续时间长。病毒载体中所含启动子的不同，其基因导致人的靶组织也不同。含茂细胞病毒启动子的病毒载体可将基因转移至视网膜色素上皮细胞，应用视紫红质启动子则转移至视细胞。

The development in the studies of virus vector-mediated gene transfer in retina

The efficient gene transfer into retina cells by virus vectors will be useful for gene therapy of retinal diseases such as retinitis pigmentosa. The gene transfer using adenovirus or adeno-associated virus as vector injected into the subretinal space of eyes can express the reporter gene in retina cells. The time of gene expression in use of adeno-associated virus was the earlier and the longer than adenovirus. Different promoter in virus vector can transfer gene into the different target tissue. For example,the virus vector containing cytomegalovirus(CMV) promoter transfer into the retina pigment epithelium cells, and use of rod opsin promoter the vector transfer gene into the photoreceptor cells. The aim of this article is to summarize the advance of recent studies including variety of virus,the ways of transduction, the evaluation of reporter gene transfer into the retina and the prospect of gene therapy of retina diseases.