龈沟液钙结合蛋白和总蛋白在实验性龈炎中的变化及相互关系

目的：研究健康牙龈向实验性龈炎发展过程中龈沟液(gingival crevicular fluid，GCF)钙结合蛋白和总蛋白水平的变化，探讨钙结合蛋白与牙龈炎之间的关系及其作为牙周炎症标志物的可能性。方法：选取11名牙龈健康、无全身疾病的受试者，在其停止口腔卫生措施后21d内(第0、7、14、21天)和恢复口腔卫生措施一周后(第28天)用滤纸条收集其GCF样本(每人4颗牙)。分别采用染料结合比色法和ELISA法测定GCF中总蛋白含量和钙结合蛋白含量。结果：在实验性龈炎形成过程中，钙结合蛋白含量逐渐升高，并且在实验第21天达到高峰，恢复口腔卫生措施后其值降低，接近基线水平；总蛋白含量的变化与钙结合蛋白的变化相似。结论：GCF钙结合蛋白含量和总蛋白含量可以较好地反映牙龈炎症的变化。

Calprotectin and total protein in gingival crevicular fluid during experimental gingivitis

Objective: To measure GCF calprotectin and total protein for evaluating whether the calprotectin could be a sensitive marker for the initial inflammation of gingiva. Methods: Eleven young male subjects with healthy gingiva, who had no systemic diseases, were selected for this study. GCF samples (4 teeth /person) were collected with strips of filter paper at baseline (0 day), on the 7 th, 14 th, 21 st days (without oral hygiene), and 28 th day (7 days after reestablishing oral hygiene) during experimental gingivitis. The amount of calprotectin was measured by ELISA. The amount of total protein was assessed with protein dye binding assay. Results: The amount of calprotectin increased during the experimental gingivitis, and reached the highest level on the 21 st day. After oral hygiene was reestablished it reduced to the level of baseline. The amount of total protein had the same tendency as calprotectin. Conclusion: The amount of calprotectin and the total protein in GCF can reflect the initial inflammation of gingiva.