Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli |
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| Authors: | Tamara B. Souza Diego M. Lozer S?nia M. S. Kitagawa Liliana C. Spano Neusa P. Silva Isabel C. A. Scaletsky |
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| Affiliation: | aDepartamento de Microbiologia, Imunologia e Parasitologia;cDisciplina de Reumatologia, Universidade Federal de São Paulo, São Paulo, Brazil;bDepartamento de Patologia, Universidade Federal do Espírito Santo, Espírito Santo, Brazil |
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| Abstract: | A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx1, and stx2 for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea. |
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