口腔鳞癌肿瘤标记物血清蛋白组学的初步研究

目的筛选口腔鳞癌及健康人血清表达差异的蛋白质,为进一步研究口腔鳞癌发生机制和建立口腔癌标记物体系奠定基础。方法收集口腔鳞癌患者外周血清17例,健康人外周血清17例,采用固相pH梯度双向凝胶电泳、质谱鉴定和生物信息学分析,筛选在口腔鳞癌患者血清中差异表达的蛋白质。结果口腔鳞癌患者和健康人血清中有7个蛋白点差异表达明显,其中2个蛋白质点分别为触珠蛋白、触珠蛋白同型前原蛋白,它们在口腔鳞癌患者的血清中均高表达。结论触珠蛋白在口腔鳞癌患者外周血和健康人外周血中的表达发生了改变。

A preliminary study on serum proteomics for tumor markers of oral squamous cell carcinoma

Objective To screen the differentially expressed proteins in serums of patients with oral squamous cell carcinoma and healthy people,and to lay a foundation for further study on genesis mechanism of oral squamous cell carcinoma and establishment of oral tumor marker system.Methods Peripheral serum samples of 17 patients with oral squamous cell carcinoma and 17 healthy people were collected.Two-dimensional gel electrophoresis with immobilized pH gradients,mass spectrometry and bioinformatics analysis were employed to screen the differentially expressed proteins in serums of patients with oral squamous cell carcinoma.Results Seven protein spots were found significantly differential expression in serums of patients with oral squamous cell carcinoma and healthy people,and two of them were haptoglobin and haptoglobin isoform 1 preproprotein,respectively,which were both highly expressed in the serums of patients with oral squamous cell carcinoma.Conclusion Expression of haptoglobin in peripheral serum is vary from patients with oral squamous cell carcinoma to healthy people.