| 系统性红斑狼疮患者静脉血BCMA和CD138~+浆细胞的研究 |
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| 引用本文： | 牛晓昶,曾照芳,贾佳,李艳林,夏季,黄恒柳,邓少丽.系统性红斑狼疮患者静脉血BCMA和CD138~+浆细胞的研究[J].重庆医学,2012,41(7):643-645. |
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| 作者姓名： | 牛晓昶 曾照芳 贾佳 李艳林 夏季 黄恒柳 邓少丽 |
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| 作者单位： | 牛晓昶 (第三军医大学大坪医院野战外科研究所检验科,重庆,400042) ； 曾照芳 (重庆医科大学检验系,400016) ； 贾佳 (重庆医科大学检验系,400016) ； 李艳林 (重庆医科大学附属儿童医院,400014) ； 夏季 (重庆医科大学检验系,400016) ； 黄恒柳 (第三军医大学大坪医院野战外科研究所检验科,重庆,400042) ； 邓少丽 (第三军医大学大坪医院野战外科研究所检验科,重庆,400042) ； |
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| 基金项目： | 国家自然科学基金资助项目,重庆市自然科学基金资助项目 |
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| 摘 要： | 目的探讨系统性红斑狼疮(SLE)患者和健康者静脉血B淋巴细胞成熟抗原(BCMA)表达和CD138+浆细胞数量的差异。方法将40例SLE患者血液标本作为SLE组,30例健康人血液标本作为对照组,采用荧光定量实时聚合酶链反应及酶联免疫吸附测定(ELISA)技术检测BCMA的表达,流式细胞技术检测静脉血CD138+浆细胞数量。结果 SLE组患者静脉血中BCMA mRNA水平、蛋白含量以及CD138+浆细胞的数量明显高于对照组(P<0.05)。结论 SLE患者中浆细胞数量的增多可能与BCMA高表达相关。
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| 关 键 词： | B淋巴细胞 浆细胞 红斑狼疮 系统性 聚合酶链反应 酶联免疫吸附测定 |
A study of BCMA and CD138~+ plasmacytoid cells in peripheral blood of patients with systemic lupus erythematosus |
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| Niu Xiaochang,Zeng Zhaofang,Jia Jia,Li Yanlin,Xia Ji,Huang Hengliu,Deng Shaoli.A study of BCMA and CD138~+ plasmacytoid cells in peripheral blood of patients with systemic lupus erythematosus[J].Chongqing Medical Journal,2012,41(7):643-645. |
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| Authors: | Niu Xiaochang Zeng Zhaofang Jia Jia Li Yanlin Xia Ji Huang Hengliu Deng Shaoli |
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| Institution: | 1(1.Department of Clinical Laboratory,Daping Hospital,Third Military Medical University,Chongqing 400042,China; 2.Department of Clinical Laboratory,Chongqing Medical University,Chongqing 400016,China; 3.Children′s Hospital of Chongqing Medical University,Chongqing 400014,China) |
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| Abstract: | Objective To study the differences of B cell maturation antigen(BCMA) expression and the amount of CD138+ plasmacytoid cells in venous blood between patients with systemic lupus erythematosus(SLE) and healthy people.Methods 40 blood samples of patients with SLE served as SLE group and 30 blood samples of healthy people served as control group.Fluorescent quantitative real time-polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA)were used to detect the expression of BCMA.Flow cytometry was adopted to assay the amount of CD138+ plasmacytoid cells in venous blood.Results The mRNA level and protein content of BCMA and the amount of CD138+ plasmacytoid cells in venous blood of patients in SLE group were significantly higher than those in control group(P<0.05).Conclusion The increased amount of plasmacytoid cells in patients with SLE may be related to the high expression of BCMA. |
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| Keywords: | B-lymphocytes plasma cells lupus erythematosus systemic polymerase chain reaction enzyme-linked immunosorbent assay |
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