Presence ofSchistosoma mansoni antigens in liver,spleen and kidney of infected mice: a sequential study

In the present study the kinetics of the uptake and deposition ofSchistosoma mansoni antigens in liver, spleen and kidney ofS. mansoni infected Swiss mice have been investigated in relation to duration of infection and infection dose (50, 100, 200 cercariae). The presence of antigen was studied with a direct immunofluorescence reaction on frozen sections of the organs, using a number of fluorescein isothiocyanate (FITC)-labeled antisera produced against various antigen preparations isolated from different life-cycle stages of the parasite. The presence of antigen was demonstrable with two of the antisera, directed against the circulating anodic antigen (CAA) and against total soluble egg antigen (SEA).CAA was demonstrable from 1 week post infection (p.i.) onwards in Kupffer cells in the liver, from 2–3 weeks p.i. onwards in macrophages in the marginal zones in the spleen and from 3 weeks onwards in kidney glomeruli. Immunofluorescence reactions on CAA in kidney glomeruli, however, were only weak positive until 12 weeks p.i., whereafter strong positive reactions were found.SEA was demonstrable from 5 weeks p.i. onwards in Kupffer cells in the liver and from 4 weeks p.i. onwards in macrophages of the spleen. In contrast to CAA, SEA was not detectable in kidney glomeruli.