纳豆激酶的体外溶栓作用研究

目的 探讨纳豆激酶（NK）的体外溶栓效果，筛选高产NK的优质菌株。方法 采用改良纤维蛋白原平板法，取不同菌株（1、3、6、8、10、11、12、13号）纳豆枯草杆菌发酵大豆．观察其发酵液、粗酶液体外6、18、24h溶解纤维蛋白原的效果（测定不同时间的溶圈直径），并与标准尿激酶（UK）的溶栓效果比较。结果发酵液和粗酶液均有溶栓作用，作用6、18h3、6、8号菌株的发酵液及粗酶液18h的溶栓作用强于尿激酶．尤其是6号菌株。结论 NK3、6、8号菌株体外溶栓作用确切；6号菌株产生的NK酶量多，且溶栓能力强、作用时间相对较长，属优质高产菌株。

Study of nattokinase thrombolysis role in vitro

[objective] To discuss the thrombolysis role of nattokinase in different natto strain to select the optimal natto strain.[Methods] Fermented liquid in different natto strain,purified liquid and urokinase in vitro dissolved the fibrinogen in different time.Measured their diameters and analyzed their effects.[Results] Either fermented liquid or purified liquid in the fibrin plate could make thrombolysis and their role was more stronger than that of urokinase.[Conlusion] The 3 th,6 th and 8 th natto strains have exact thrombolysis role,nattokinase produced by 6th natto strain is the most,it's thrombolysis effect is good and thrombolysis time is longer than the others.The 6th strain is an optimal natto strain with high-quality and more-products.