Transformation of Trichoderma species with dominant selectable markers |
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Authors: | Cirano J Ulhoa Marilene H Vainstein John F Peberdy |
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Institution: | (1) Department of Life Science, Microbial Biochemistry and Genetics Group, School of Biological Sciences, University Park, NG7 2RD Nottingham, England;(2) Present address: Laboratorio de Microbiologia, Departamento de Biologia Celular, Universidade de Brasilia, Brasilia, DF, Brazil |
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Abstract: | Summary We have developed a transformation system for Trichoderma hamatum and Trichoderma harzianum Rifai, using dominant markers for selection based on the Escherichia coli hygromycin B phosphotransferase gene (hph) and the -tubulin gene (bml) from Neurospora crassa, respectively. Transformation frequencies and protoplast regeneration were low in both species. All the T. hamatum hygromycin-resistant transformants analysed were mitotically stable, in contrast to those of T. harzianum derived by benomyl resistance, in which only 50% of the transformants analysed were stable. Molecular analysis of transformants showed the integration of the transforming DNA into the genome and indicated that the number and sites of integration varied among the transformants. |
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Keywords: | Trichoderma Transformation Hygromycin B resistance Benomyl resistance |
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