酒精的发育毒性靶器官探讨

目的 探讨酒精发育毒性和致畸机制。方法 于体外施与孕９．５ｄ大鼠胚胎不同剂量酒精,应用植入后全胚胎培养和荧光偏振技术研究酒精对胚胎发育影响及酒精对卵黄囊（ＶＹＳ）细胞膜脂质流动性影响。结果 ４００ｍｇ／Ｌ酒精对胚胎发育、器官形态分化各指标及ＶＹＳ细胞膜脂质荧光偏振度（Ｐｒ）和流动度（ＬＦＵ）与对照组比较,差异无显性,１０００ｍｇ／Ｌ组脑、ＶＹＳ直径、ＶＹＳ循环、神经管、头长、心脏、ＤＮＡ含量和Ｐ

Studies on the mechanisms of developmental toxicity of alcohol

Objective To study the mechanisms of developmental toxicity in animals induced by ethyl al cohol. Method Rat embryos of 9 5 days of gestation were exposed to di fferent doses of ethyl alcohol in vitro. Postimplantation whole embryo culture a nd fluorescence polarization technique were used to investigate the effects of a lcohol on embryo development and visceral yolk sac (VYS) membrane lip id fluidity. Result 400 mg alcoho/L caused no significant differences to emb ryo development, organ morphological scores, polarization (Pr value) and lipid f luidity unit (LFU), as compared with the non-alcohol treated controls. 1 000 mg alcohol/L led to the decrease of scores of brain, VYS circle, neurotube, head le ngth and heart, as well as DNA and Pr value; whereas LFU values were increased s igni ficantly. Above 2 000 mg alcohol/L, all indexes were decreased except LFU of VYS cell membrane. Alcohol significantly elevated lipid fluidity of VYS cell membra ne. Conclusions Alcohol causes development toxicity and teratog enisis in rats. Brain and VYS are the targets of alcohol effect. Its development toxicity is related with lipid fluidity and the VYS membrane damage.