分泌型卷曲相关蛋白4、微小RNA-126-5p在三阴乳腺癌组织中表达及与临床病理特征的关系

目的 探究分泌型卷曲相关蛋白4(SFRP4)、微小RNA-126-5p在三阴乳腺癌组织中表达及与临床病理特征的关系。方法 选取2014年10月至2016年10月在沧州市人民医院接受手术切除治疗的三阴乳腺癌病人78例作为研究对象，取其癌组织及癌旁组织（距离肿瘤边缘5 cm），以SP免疫组化法检测SFRP4表达情况，以RT-qPCR分析法检测miR-126-5p水平。收集病人资料，分析SFRP4、miR-126-5p表达与三阴乳腺癌病人临床病理特征的关系。以Spearman等级相关性分析SFRP4、miR-126-5p表达的相关性。绘制K-M生存曲线分析病人生存状态，行Cox多因素分析影响三阴乳腺癌病人预后的因素。结果 三阴乳腺癌组织中SFRP4阳性率53.85%(42/78)]明显高于癌旁组织37.18%(29/78)](P<0.05),miR-126-5p表达0.78±0.21明显低于癌旁组织1.00±0.00(P<0.05)。对纳入病人进行临床病理特征分析，结果显示，SFRP4表达与淋巴结转移78.13%(25/32)比36.96%(17/46)]、肿瘤浸润程度6...

Expression of secreted frizzled-related protein 4 and miR-126-5p in triple-negative breast cancer tissues and their relationship with clinicopathological characteristics

Objective To explore the expression of secreted frizzled-related protein 4 (SFRP4) and miR-126-5p in triple-negative breast cancer tissues and their relationship with clinicopathological characteristics.Methods Seventy-eight patients with triple-negative breast cancer who underwent surgical resection in our hospital were selected as the research objects. The cancer tissues and adjacent tissues (5 cm from the tumor edge) were taken, SP immunohistochemistry was used to detect the expression of SFRP4, and RT-qP?CR analysis method was used to detect the level of miR-126-5p. The data of patients were collected, and the relationship between the expression of SFRP4, miR-126-5p and the clinicopathological characteristics of triple-negative breast cancer patients was analyzed.Spearman rank correlation was used to analyze the correlation between SFRP4 and miR-126-5p expression. K-M survival curve was plotted to analyze the survival status of patients, Cox multivariate analysis was performed to analyze the factors affecting the prognosisof patients with triple-negative breast cancer.Results The positive rate of SFRP4 in triple-negative breast cancer tissues 53.85% (42/ 78)] was significantly higher than that in adjacent tissues 37.18% (29/78)] (P<0.05), and the expression of miR-126-5p (0.78±0.21) was significantly lower than that in adjacent tissues (1.00±0.00) (P<0.05). The clinical pathological characteristics of the included patients were analyzed, and the results showed that the expression of SFRP4 was related to lymph node metastasis 78.13% (25/32) vs. 36.96% (17/46)], tumor invasion 65.12% (28/43) vs. 22.86% (8/35)] and Ki-67 expression 83.33% (20/24) vs. 40.74% (22/54)] (P< 0.05), but not related to age, tumor size, TNM stage and histological grade (P>0.05). MiR-126-5p was related to lymph node metastasis 81.25% (26/32) vs. 30.43% (14/46)] and Ki-67 expression 70.83% (17/24) vs. 42.59% (23/54)] (P<0.05), but not related to age, tumor size, TNM stage, histological grade, and tumor invasion (P>0.05). Spearson correlation analysis was performed, and the results showed that SFRP4 and miR-126-5p were correlated in triple-negative breast cancer tissues (P<0.05). The patients were followed up for 5 years, 63 cases survived and 15 died. In the miR-126-5p low expression group, 28 patients survived and 12 died, while in the miR-1265p high expression group, 35 patients survived and 3 died, and the difference between the two groups was statistically significant (P< 0.05); in the SFRP4 positive group, 29 patients survived and 13 died, while in the SFRP4 negative group, 34 patients survived and 2died, and the difference between the two groups was statistically significant (P<0.05). Multivariate Cox analysis showed that lymph node metastasis, Ki-67 positive, low expression of miR-126-5p and positive SFRP4 were independent risk factors affecting the prognosis of patients with triple-negative breast cancer (P<0.05).Conclusion The expression of miR-126-5p in triple-negative breast cancertissue is low, and the positive rate of SFRP4 is high. Their expression is correlated and can be used as an important indicator for predicting the prognosis of patients with triple-negative breast cancer.