The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin |
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| Authors: | Arzu Tezvergil-Mutluay Kelli A. Agee Tomohiro Hoshika Franklin R. Tay David H. Pashley |
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| Affiliation: | 1. Department of Prosthetic Dentistry, Institute of Dentistry, University of Turku, Turku, Finland;2. Department of Oral Biology, Medical College of Georgia, School of Dentistry, Augusta, GA, USA;3. Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;4. Department of Endodontics, Medical College of Georgia, School of Dentistry, Augusta, GA, USA;1. Bauru School of Dentistry, University of São Paulo, Department of Oral Biology, 9-75 Al. Octávio Pinheiro Brisolla, Bauru, SP 17012-901, Brazil;2. University of Sagrado Coração, Department of Dentistry, 10-50 Irmã Arminda ST, Bauru, SP 17011-160, Brazil;3. Institute of Dentistry, University of Oulu, and Medical Research Center Oulu, Oulu University Hospital, PO Box 5281, 90014 Oulu, Finland;1. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, IL, USA;2. Department of Dental Materials and Prosthodontics, Ribeirão Preto Dental School, University of São Paulo, SP, Brazil;1. Department of Postgraduate Program in Dentistry, CEUMA University, São Luis, MA, Brazil;2. Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, PR, Brazil;3. Institute for Research in Dental Sciences, Faculty of Dentistry, University of Chile, Chile;4. Dentistry Academic Unit, Faculty of Medicine, Pontificia Universidad Católica de Chile, Chile;5. Department of Oral & Craniofacial Sciences, University of Missouri—Kansas City, Kansas City, MO, USA;1. Adhesive Dentistry Research Group, Institute of Dentistry, University of Turku, Turku, Finland;2. Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA;3. Department of SAU&FAL, University of Bologna, Bologna, Italy;4. Department of Oral Science, School of Dentistry, University of British Columbia, Vancouver, British Columbia, Canada;5. UNIBAN (University Bandeirante Anhanguera), Biomaterials Research Group, Sao Paulo, Brazil;6. Centro Interdisciplinar de Investigacão Bioquimica, University of Mogi das Cruzes, Mogi das cruzes, Brazil;7. Department of Biochemistry, Federal University São Paulo, Brazil;8. Biomaterials Research Group, UNIBAN, São Paulo, Brazil;9. Osaka University Graduate School of Dentistry, Department of Biomaterials Science, Osaka, Japan;10. Institute of Dentistry, University of Oulu, Oulu University Hospital, Oulu, Finland;11. Institute of Dentistry, University of Turku, Turku, Finland;1. Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil;2. Department of Operative Dentistry, Universidade Paranaense, Cascavel, PR, Brazil;3. Department of Operative Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil;4. Institute of Dentistry, University of Oulu, and Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland;5. Department of Oral and Maxillofacial Diseases, University of Helsinki, and Helsinki University Hospital, Helsinki, Finland;6. Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil;7. Department of Pediatric Dentistry, Orthodontics and Collective Health Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, SP, Brazil;1. Institute of Dentistry, University of Oulu/Oulu University Hospital, Oulu, Finland;2. Institute of Dentistry, University of Turku, Turku, Finland;3. The NordicInstitute of Dental Materials (NIOM), Oslo, Norway;4. Biomaterials Research Group – UNIBAN, São Paulo, Brazil;5. Department of Medical Sciences, Unit of Dental Sciences and Biomaterials, University of Trieste, Italy;6. IGM-CNR, Unit of Bologna c/o IOR, Bologna, Italy;7. Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, São Paulo, Brazil;8. Universidade Federal de São Paulo (UNIFESP), Disciplina de Biologia Molecular, São Paulo, Brazil;9. Restorative Dental Sciences Operative Division, College of Dentistry, University of Florida, Gainesville, FL, USA;10. Department of Prosthetic Dentistry, Institute of Dentistry, University of Turku, Finland;11. Schulich Dentistry, University of Western Ontario, London, ON, Canada;12. Department of Oral Biological and Medical Sciences, Division of Biomaterials, University of British Columbia, Vancouver, Canada;13. Department of Endodontics, Medical College of Georgia, School of Dentistry, Augusta, GA, USA;14. Department of Oral Biology, Medical College of Georgia, School of Dentistry, Augusta, GA, USA |
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| Abstract: | This study has examined the use of polyvinylphosphonic acid (PVPA) as a potential matrix metalloproteinase (MMP) inhibitor and how brief cross-linking of demineralized dentin matrix that did not affect its mechanical properties enhanced the anti-MMP activity of PVPA. The anti-MMP potential of five PVPA concentrations (100–3000 μg ml–1) was initially screened using a rhMMP-9 colorimetic assay. Demineralized dentin beams were treated with the same five concentrations of PVPA to collagen and then aged for 30 days in a calcium- and zinc-containing medium. The changes in modulus of elasticity, loss of dry mass and dissolution of collagen peptides were measured via three-point bending, precision weighing and hydroxyproline assay, respectively. All tested PVPA concentrations were highly effective (P < 0.05) in inhibiting MMP-9. Ageing in the incubation medium did not significantly alter the modulus of elasticity of the five PVPA treatment groups. Conversely, aged dentin beams from the control group exhibited a significant decline in their modulus of elasticity (P < 0.05) over time. Mass loss from the dentin beams and the corresponding increase in hydroxyproline in the medium in the five PVPA treatment groups were significantly lower than for the control (P < 0.05). PVPA is a potent inhibitor of endogenous MMP activities in demineralized dentin. It may be used as an alternative to chlorhexidine to prevent collagen degradation within hybrid layers to extend the longevity of resin–dentin bonds. |
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