Remarkably enhanced stability and function of core/shell nanoparticles composed of a lecithin core and a pluronic shell layer by photo-crosslinking the shell layer: In vitro and in vivo study |
| |
Authors: | Won Il Choi Kyung Chul Yoon Seong Kyu Im Young Ha Kim Soon Hong Yuk Giyoong Tae |
| |
Institution: | 1. School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China;2. School of Pharmaceutical Science, Liaoning University, 66 ChongShan Mid Road, Shenyang 110036, China;1. Chemical Engineering, Oklahoma State University, 420 Engineering North, Stillwater, OK 74078, United States;2. Polymer Engineering Department, Faculty of Engineering, Urmia University, Urmia, Iran;3. School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran;4. Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran;5. Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences, University of Tehran, Tehran, Iran;6. Marquette University School of Dentistry, Milwaukee, WI 53233, United States;7. Department of Bioinformatics, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran;8. National Cell Bank of Iran, Pasteur Institute of Iran, P.O. Box 1316943551, Tehran, Iran;9. Department of Polymer Technology, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland;10. Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran;11. Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran;12. School of Chemical Sciences, M G University, Kottayam 686560, Kerala, India;1. Dept. of Scienze della Vita e dell''Ambiente, University of Cagliari, Cagliari, Italy;2. Dept. of Drug chemistry and technologies, Sapienza, University of Roma, Roma, Italy;3. Dept. of Pharmacy and Pharmaceutical Technology and Parasitology, University of Valencia, Burjassot, Valencia, Spain;4. Institute of Molecular Recognition and Technological Development, Inter-University Institute from Polytechnic University of Valencia and University of Valencia, Spain;5. Dept. of Pharmacology, University of Valencia, Burjassot, Valencia, Spain;6. Interdisciplinary Center for Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne, Station 12, Lausanne, Switzerland;7. Dept. of Biochemistry and Molecular Biology A, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia, Spain |
| |
Abstract: | A core/shell nanoparticle system with a lecithin core and a pluronic shell has been previously reported, and it was shown to act as an effective sustained release system for positively charged proteins. Here, to provide improved stability of the core/shell nanoparticle system in a physiological environment, we prepared the core/shell nanoparticle system with a photo-crosslinked shell layer by using a lecithin liposome as the core and pluronic F 127 diacrylate (DA-PF 127) as the shell layer. The DA-PF 127 was then photo-polymerized. Compared with a purely physical system, chemical crosslinking of the shell layer resulted not only in significantly increased structural stability of the core/shell nanoparticles in both an organic co-solvent and in serum but also several remarkably enhanced functioning as a protein delivery system. First, the chemically crosslinked systems were resuspended in aqueous solution after lyophilization without using a cryo-protectant. Second, target proteins were efficiently loaded into the nanoparticles by simple co-incubation in aqueous solution at a low temperature (4 °C) and the dried powder form of the protein-loaded nanoparticles was obtained. The loading capacity of the system was increased by more than 10 times compared with that of a purely physical system. Most importantly, the chemically crosslinked system showed more sustained release of the loaded proteins, and the release rate was not noticeably affected by the presence of serum proteins, whereas sustained release of loaded vascular endothelial growth factor (VEGF) in a purely physical system was greatly reduced by serum proteins. In an in vivo corneal angiogenesis assay the chemically crosslinked system loaded with VEGF resulted in more efficient new blood vessel formation than the physical system. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|