Encapsulation of fibroblasts causes accelerated alginate hydrogel degradation |
| |
| Authors: | NC Hunt AM Smith U Gbureck RM Shelton LM Grover |
| |
| Institution: | 1. School of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK;2. Department of Functional Materials in Medicine and Dentistry, University of Wurzburg, Wurzburg D97070, Germany;3. School of Dentistry, University of Birmingham, Birmingham B4 6NN, UK;1. Biotechnology-Medical Science, KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 136-701, Republic of Korea;2. Department of Biomedical Engineering, College of Health Science, Korea University, Seoul 136-703, Republic of Korea;1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, PR China;2. College of Materials and Textile Engineering, Jiaxing University, Zhejiang 314001, PR China;1. Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea;2. Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea;1. Centre for Rapid and Sustainable Product Development, Polytechnic Institute of Leiria, Centro Empresarial da Marinha Grande, Marinha Grande, Portugal;2. Chemical Engineering Department, Faculty of Science and Technology of University of Coimbra, Pólo2, Coimbra, Portugal |
| |
| Abstract: | Calcium-alginate hydrogel has been widely studied as a material for cell encapsulation for tissue engineering. At present, the effect that cells have on the degradation of alginate hydrogel is largely unknown. We have shown that fibroblasts encapsulated at a density of 7.5 × 105 cells ml?1 in both 2% and 5% w/v alginate remain viable for at least 60 days. Rheological analysis was used to study how the mechanical properties exhibited by alginate hydrogel changed during 28 days in vitro culture. Alginate degradation was shown to occur throughout the study but was greatest within the first 7 days of culture for all samples, which correlated with a sharp release of calcium ions from the construct. Fibroblasts were shown to increase the rate of degradation during the first 7 days when compared with acellular samples in both 2% and 5% w/v gels, but after 28 days both acellular and cell-encapsulating samples retained disc-shaped morphologies and gel-like spectra. The results demonstrate that although at an early stage cells influence the mechanical properties of encapsulating alginate, over a longer period of culture, the hydrogels retain sufficient mechanical integrity to exhibit gel-like properties. This allows sustained immobilization of the cells at the desired location in vivo where they can produce extracellular matrix and growth factors to expedite the healing process. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
