A novel modular device for 3-D bone cell culture and non-destructive cell analysis |
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| Authors: | Friederike Kunz Claudia Bergemann Ernst-Dieter Klinkenberg Arne Weidmann Regina Lange Ulrich Beck J. Barbara Nebe |
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| Affiliation: | 1. University of Rostock, Biomedical Research Centre, Department of Cell Biology, Schillingallee 69, D-18057 Rostock, Germany;2. DOT GmbH, Rostock, Charles Darwin-Ring 1a, D-18059, Germany;3. University of Rostock, Department of Electrical Engineering and Informatics, A.-Einstein-Str. 2, D-18051 Rostock, Germany;1. Àrea de Prehistoria, Universitat Rovira i Virgili (URV), Avinguda de Catalunya 35, 43002 Tarragona, Spain;2. IPHES, Institut Català de Paleoecologia Humana i Evolució Social, C/Marcel·lí Domingo s/n Edifici W3, 43007 Tarragona, Spain;3. GQP-CG, Grupo Quaternário e Pré-História do Centro de Geociências (uI&D 73 – FCT), Portugal;1. Leibniz-Institute of Atmospheric Physics at the University of Rostock, Kühlungsborn 18225, Germany;2. Department of Physics, University of Colorado, Boulder, CO 80309, USA;3. Graz University of Technology, 8010 Graz, Austria;4. Department of Meteorology, Stockholm University, 106 91 Stockholm, Sweden;1. Space Science Division, Naval Research Laboratory, Washington, DC, United States;2. NASA Langley Research Center, Hampton, VA, United States;3. GATS Inc., Hampton, VA, United States;4. Graz University of Technology, Graz, Austria;5. MISU, Stockholm University, Stockholm, Sweden;1. Department of Respiratory Medicine, M.M Institute of Medical Sciences & Research, Mullana, Ambala, Haryana, India;2. Department of Biochemistry, M.M Institute of Medical Sciences & Research, Mullana, Ambala, Haryana, India;1. Universität Tübingen, Department of Geosciences, Biogeology, Hölderlinstrasse 12, D-72074 Tübingen, Germany;2. Zoological Institute, Russian Academy of Sciences, Universitetskaya nab. 1, Saint Petersburg 199034, Russia;3. Royal Belgian Institute of Natural Sciences, Vautierstraat 29, B-1000 Brussels, Belgium;4. University of Leuven, Laboratory of Biodiversity and Evolutionary Genomics, Ch. Debériotstraat 32, B-3000 Leuven, Belgium |
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| Abstract: | Synthetic materials have emerged as bone substitutes for filling bone defects of critical sizes. Because bone healing requires a mechanically resistant matrix (scaffold) attractive to osteogenic cells and must allow revascularization for nutrient and oxygen supply, scaffold-based strategies focus on the further development of chemical and physical qualities of the material. Cellular ingrowth towards the scaffold center is critical; therefore selective information from inner regions, in particular from the central part, is essential.In this paper we introduce a novel modular in vitro system for three-dimensional (3-D) in vitro bone cell cultures. This 3-D system is developed exclusively for in vitro research purposes, with special emphasis on the geometrical scaffold design (pore size, pore design). The system is composed of a stack of titanium slices which are mounted on a clamp and which enable the separate monitoring of cell growth patterns on every single slice of the slide stack. In this way we are able to gain selective information about the regulation of the cell physiology in the inner part of the 3-D construct which can be used for the development of an optimized scaffold design for orthopedic implants. |
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