Blood compatibility of photografted hydrogel coatings |
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| Authors: | Lars Faxälv Tobias Ekblad Bo Liedberg Tomas L. Lindahl |
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| Affiliation: | 1. Department of Clinical and Experimental Medicine, Division of Clinical Chemistry, Linköping University, SE-581 85 Linköping, Sweden;2. Department of Physics, Chemistry and Biology, Division of Molecular Physics, Linköping University, SE-581 83 Linköping, Sweden;1. Department of Chemical and Biological Engineering, University of Colorado at Boulder, Boulder, CO 80309, USA;2. Fairview High School, Boulder, CO 80305, USA;3. Howard Hughes Medical Institute and the BioFrontiers Institute, University of Colorado at Boulder, Boulder, CO 80309, USA;1. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA;2. Materials Science Program, University of Wisconsin-Madison, WI, USA;3. Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI, USA;1. Institute Biofunctional Polymer Materials, Max Bergmann Center of Biomaterials, Leibniz-Institut für Polymerforschung Dresden e.V., Dresden, Germany;2. i3S, Instituto de Investigação e Inovação em Saúde, Portugal;3. INEB, Instituto de Engenharia Biomédica, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal;4. ICBAS, Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal;5. Institut für Biomedizinische Technik, Universitätsmedizin Rostock, Friedrich-Barnewitz-Str. 4, 18119 Rostock, Germany;6. Institute for ImplantTechnology and Biomaterials (IIB) e.V., Friedrich-Barnewitz-Str. 4, 18119 Rostock, Germany;7. Key Laboratory of Advanced Technology for Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;8. Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115, United States;9. Wyss Institute for Biologically Inspired Engineering at Harvard University, 3 Blackfan Circle, Boston, MA 02115, United States;10. Harvard-MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States |
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| Abstract: | In this work, we have evaluated the haemocompatibility of different surface modifications, intended for biomaterials and bioanalytical applications. Polystyrene slides were coated with thin hydrogel films by self-initiated photografting and photopolymerization (SIPGP) of four different monomers. The hydrogel surface modifications were thoroughly characterized and tested for their protein resistance and ability to resist platelet adhesion and activation of the coagulation system. There was very little protein adsorption from human plasma on the hydrogels prepared from poly(ethylene glycol) methacrylate and 2-hydroxyethyl methacrylate. Platelet adhesion tests performed under both static and flow conditions showed that these coatings also demonstrated very high resistance towards platelet adhesion. A small amount of platelets were found to adhere to hydrogels formed from ethylene glycol methyl ether methacrylate and 2-carboxyethyl methacrylate. The polystyrene substrates themselves facilitated large amounts of platelet adhesion under both static and flow conditions. Utilizing a novel setup for imaging of coagulation, it was confirmed that none of the hydrogel surfaces activated the coagulation system to any great extent. We suggest that this simple fabrication method can be used to produce hydrogel coatings with unusually high blood compatibility, suitable for demanding biomaterials applications. |
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