Interaction of an ordered mesoporous bioactive glass with osteoblasts,fibroblasts and lymphocytes,demonstrating its biocompatibility as a potential bone graft material |
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| Authors: | M Alcaide P Portolés A López-Noriega D Arcos M Vallet-Regí MT Portolés |
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| Institution: | 1. Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain;2. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain;3. Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, Universidad Complutense, 28040 Madrid, Spain;4. Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, Madrid, Spain;1. Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, 330-714, Republic of Korea;2. Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea;3. Regenerative Medicine Research Institute, Universitat Internacional de Catalunya Barcelona 08017, Spain;4. Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, 330-714, Republic of Korea;1. Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing, China;2. Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China;3. National Engineering Research Center for Human Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China;1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea;2. Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea;3. Glass Research Department, National Research Center, Cairo 12622, Egypt;4. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea;1. Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, P.O. Box 917794-8564, Mashhad, Iran;2. Center for Research, Technology and Education in Vitreous Materials (CeRTEV), Federal University of São Carlos, 13565-905 São Carlos, SP, Brazil;3. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, 14155-6447 Tehran, Iran;4. Medical Genetics Network (MeGeNe), Universal Scientific Education and Research Network (USERN), Tehran, Iran;5. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 31116, South Korea;6. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 31116, South Korea;7. Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine Research Center, Dankook University, Cheonan 31116, South Korea;8. Institute of Materials Physics and Engineering, Applied Science and Technology Department, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy;1. Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain;2. CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Madrid, Spain;3. Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 28040 Madrid, Spain |
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| Abstract: | Ordered mesoporous 85SiO2–10CaO–5P2O5 bioactive glass (MBG85) is an excellent candidate as a graft for bone tissue regeneration, owing to its excellent textured properties, structural characteristics and crystalline apatite rate formation. To assess MBG85 biocompatibility, different parameters have been evaluated (cell morphology, size/complexity, proliferation, viability, cell cycle, reactive oxygen species content, lactate dehydrogenase release) using human Saos-2 osteoblasts after treatment with either MBG85 extracts or 1% MBG85 directly added to cells. The osteoblast response to MBG85 was compared with L929 fibroblast behaviour after the same treatment. The high cell viability observed and the absence of signs of cell damage in both cell types demonstrates MBG85 biocompatibility. Only a cytostatic effect was observed through the reduction of cell proliferation, related with the initial Ca elution, whereas Si leaching did not result into any negative effect. In vitro lymphocytic proliferation analysis was also carried out with SR.D10 clone after treatment with either MBG85 extracts or culture supernatants of L929 fibroblasts previously treated with 1% MBG85 (cell-conditioned extracts). The absence of modification of in vitro T-cell response underlines the biocompatibility of MBG85 and its potential application in the field of bone and dental grafting. |
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