Surface modification of and selective protein attachment to a flexible microarray pattern using atmospheric plasma with a reactive gas |
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| Authors: | Hyun-Uk Lee So-Young Park Yoon-Hee Kang Se-Young Jeong Sae-Hae Choi Kwang-Yeop Jahng Chae-Ryong Cho |
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| Affiliation: | 1. Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Jiaotong University, China;2. Department of Orthopedic Surgery, Shanghai Jiao Tong University affiliated Sixth People''s Hospital, Shanghai Jiaotong University, China |
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| Abstract: | The hydrophobic and hydrophilic properties of the surface of poly-ether sulfone (PES) films were controlled by an atmospheric pressure plasma (AP) treatment using reactive gases (Ar/H2 and Ar/O2). The surface properties of the Ar/H2 and Ar/O2 in series AP-treated PES films showed higher surface roughness (～120%), surface energy (～30%) and hydrophilic properties (oxygen content ～10%) than the Ar/O2 AP-treated PES film. The protein staining results confirmed that an activated region on the patterned PES film with high selectivity and sensitivity was well-defined and formed. This method is suitable for fabricating flexible protein adhesive chips with uniform biomolecular adhesive properties. |
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