The effect of complexation hydrogels on insulin transport in intestinal epithelial cell models |
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| Authors: | Kristy M Wood Gregory M Stone Nicholas A Peppas |
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| Institution: | 1. Biomedical Engineering, University of Texas at Austin, 1 University Station C0400, Austin, TX 78712, USA;2. Chemical Engineering, University of Texas at Austin, 1 University Station C0400, Austin, TX 78712, USA;3. Division of Pharmaceutics, University of Texas at Austin, 1 University Station C0400, Austin, TX 78712, USA;1. Laboratory of Disease Genomics and Individualized Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. College of Life Sciences Inner Mongolia University, Hohhot 010021, China;4. Science and Technology Department, People’s Government of Inner Mongolia Autonomous Region, Hohhot 010010, China;1. Institute of Geological Sciences, Polish Academy of Sciences, Research Centre in Warsaw, INGPAN, Twarda St. 51/55, PL-00-818 Warsaw, Poland;2. Institute of Geography and Regional Studies Pomeranian University in S?upsk, Partyzantów St. 27, PL-76-200 S?upsk, Poland;3. Institute of Biology, University of Bia?ystok, Cio?kowskiego St. 1J, PL-15-245 Bia?ystok, Poland;1. Department of Medical Biophysics, Medical Research Institute, Alexandria University, Egypt;2. Department of Pharmaceutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Egypt;1. Department of Mathematics, COMSATS Institute of Information Technology, Park Road, Chak Shahzad, Islamabad 44000, Pakistan;2. Department of Mathematics, Pennsylvania State University, York Campus, Edgecomb 1703, USA;3. Department of Mathematics, COMSATS Institute of Information Technology, Abbottabad, Pakistan |
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| Abstract: | A novel class of pH-sensitive complexation hydrogels composed of methacrylic acid and functionalized poly(ethylene glycol) (PEG) tethers, referred to as P(MAA-g-EG) WGA, was investigated as an oral protein delivery system. The PEG tethers were functionalized with wheatgerm agglutinin (WGA), a lectin that can bind to carbohydrates in the intestinal mucosa, to improve residence time of the carrier and absorption of the drug at the delivery site. The ability of P(MAA-g-EG) WGA to improve insulin absorption was observed in two different intestinal epithelial models. In Caco-2 cells P(MAA-g-EG) WGA improved insulin permeability 9-fold as compared with an insulin only solution, which was similar to the improvement by P(MAA-g-EG). P(MAA-g-EG) and P(MAA-g-EG) WGA were also evaluated in a mucus-secreting culture that contained Caco-2 and HT29–MTX cells. Insulin permeability was increased 5-fold in the presence of P(MAA-g-EG) and P(MAA-g-EG) WGA. Overall, it is clear that P(MAA-g-EG) WGA enhances insulin absorption and holds great promise as an oral insulin delivery system. |
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