The effect of topology of chitosan biomaterials on the differentiation and proliferation of neural stem cells |
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| Authors: | Gan Wang Qiang Ao Kai Gong Aijun Wang Lu Zheng Yandao Gong Xiufang Zhang |
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| Affiliation: | 1. State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, People’s Republic of China;2. Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University, Beijing 100049, People’s Republic of China;3. Department of Bioengineering, University of California, Berkeley, B104 Stanley Hall, Berkeley, CA 94720, USA;1. Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA;2. Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA;3. Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, PR China;4. Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA;1. Department of Ceramics and Glass Engineering and CICECO, University of Aveiro, 3810-193 Aveiro, Portugal;2. Departamento de Fisica, I3N, Universidade de Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal;3. Department of Mechanical Engineering and Centre for Mechanical Technology & Automation, University of Aveiro, 3810-193 Aveiro, Portugal;4. Ferroelectric Research Laboratory, Department of Physics, A N College, Patna 800013, India;1. Institute of Physics, University Münster, Germany;2. Max-Planck-Institute for Molecular Biomedicine, Münster, Germany;1. Krebs Institute, The University of Sheffield, Sheffield S10 2TN, UK;2. Department of Biomedical Sciences, The University of Sheffield, Sheffield S10 2TN, UK;3. Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA;4. Material Science Program, University of California, San Diego, La Jolla, CA 92093, USA;5. Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA;6. Department of Chemistry, University College London, London WC1H 0AJ, UK;7. The MRC/UCL Centre for Medical Molecular Virology, University College London, London WC1H 0AJ, UK |
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| Abstract: | Neural stem cells (NSCs) are capable of self-renewal and differentiation into three principle central nervous system cell types under specific local microenvironments. Chitosan films (Chi-F), chitosan porous scaffolds (Chi-PS) and chitosan multimicrotubule conduits (Chi-MC) were used to investigate their effects on the differentiation and proliferation of NSCs isolated from the cortices of fetal rats. In the presence of 10% fetal bovine serum most NSCs cultured on Chi-F differentiated into astrocytes, NSCs cultured on Chi-MC showed a significant increase in neuronal differentiation, while Chi-PS somewhat promoted NSCs to differentiate into neurons. However, in serum-free medium with 20 ng ml?1 basic fibroblast growth factor NSCs cultured on Chi-F showed the greatest proliferation, NSCs cultured on Chi-MC showed moderate cell proliferation, but NSCs cultured on Chi-PS exhibited the least cell proliferation. These observations indicate that chitosan topology can play an important role in regulating differentiation and proliferation of NSCs and raise the possibility of the utilization of chitosan in various structural biomaterials in neural tissue engineering. |
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