微量液体克隆形成法体外检测三尖杉酯碱、高三尖杉酯碱对CFU—GM及L—CFU的细胞毒作用

基于琼脂半固体培养法和极限稀释微孔池培养法,建立了一种新的体外药敏检测体系——微量液体克隆形成法。并应用该体系检测了三尖杉酯碱(H)和高三尖杉酯碱(HH)对CFU—GM及L—CFU的细胞毒作用。结果提示:24h及168h作用法时,HH对CFU—GM以及L—CFU的细胞毒作用均大于H,H、HH对L—CFO的细胞毒作用选择性均大于CFU—GM,但HH的细胞毒作用选择性不及H;H、HH的TDI(Time—schedule Depne—dence Index),IC_(90-24h)÷IC_(-90-168h)分别为68、60,说明两药物均为细胞周期时相特异性药物;并对H、HH之间可能存在的同系物间的交叉耐药性作了探讨。

Testing of cytet■xicity of harringtonine and homoharringtonine on CFU-GM and L - CFU with microliquid clonogenic assay in vitro

Based on semisolid agar culture and limiting diluting micro - culture, we have established a new chemosensitivity test system in vitro - microliquid clonogenic assay. Using this assay, the cytotoxicity of harringtonine ( H ) and homoharringtonine (HH) on CFU- GM (colony - forming units of granulomacrophages ) and L - CFU (leukemic colony - forming units) were teste-d. The result suggested that the cytotoxicity of HH on CFU -GM and L -CFU was more potent than that of H under 24h and 168h expo- sure methods; that cytotoxic selectivity of H and HH on L -CFU was larger than on CFU - GM, but the cytotoxic selectivity of HH was smaller than that of H. H and HH were cell cycle phasespecific drugs according to their TDIs. The possibility of homologous drug cross - resistance between H and HH were investigated.