Adiponectin induces insulin secretion in vitro and in vivo at a low glucose concentration |
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Authors: | M Okamoto M Ohara-Imaizumi N Kubota S Hashimoto K Eto T Kanno T Kubota M Wakui R Nagai M Noda S Nagamatsu T Kadowaki |
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Institution: | (1) Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan;(2) Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan;(3) Division of Applied Nutrition, National Institute of Health and Nutrition, Tokyo, Japan;(4) Department of Physiology, Hirosaki University School of Medicine, Hirosaki, Japan;(5) Department of Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan;(6) Department of Diabetes and Metabolic Medicine, International Medical Center of Japan, Tokyo, Japan |
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Abstract: | Aims/hypothesis A decrease in plasma adiponectin levels has been shown to contribute to the development of diabetes. However, it remains uncertain
whether adiponectin plays a role in the regulation of insulin secretion. In this study, we investigated whether adiponectin
may be involved in the regulation of insulin secretion in vivo and in vitro.
Methods The effect of adiponectin on insulin secretion was measured in vitro and in vivo, along with the effects of adiponectin on
ATP generation, membrane potentials, Ca2+ currents, cytosolic calcium concentration and state of 5′-AMP-activated protein kinase (AMPK). In addition, insulin granule
transport was measured by membrane capacitance and total internal reflection fluorescence (TIRF) analysis.
Results Adiponectin significantly stimulated insulin secretion from pancreatic islets to approximately 2.3-fold the baseline value
in the presence of a glucose concentration of 5.6 mmol/l. Although adiponectin had no effect on ATP generation, membrane potentials,
Ca2+ currents, cytosolic calcium concentrations or activation status of AMPK, it caused a significant increase of membrane capacitance
to approximately 2.3-fold the baseline value. TIRF analysis revealed that adiponectin induced a significant increase in the
number of fusion events in mouse pancreatic beta cells under 5.6 mmol/l glucose loading, without affecting the status of previously
docked granules. Moreover, intravenous injection of adiponectin significantly increased insulin secretion to approximately
1.6-fold of baseline in C57BL/6 mice.
Conclusions/interpretation The above results indicate that adiponectin induces insulin secretion in vitro and in vivo.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorised users.
M. Okamoto and M. Ohara-Imaizumi contributed equally to this study. |
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Keywords: | Adiponectin Beta cell Capacitance Fusion events Insulin granules Insulin secretion Islet |
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