依地福新对Hela细胞生长的抑制作用及其机制研究

目的:观察依地福新对人宫颈癌Hela细胞的体外生长抑制作用,并进一步探讨其作用机制。方法:细胞中分别加入不同浓度的依地福新(0.5、1.0、5.0、10.0μmol.L-1)处理96h,并设立未加依地福新的对照组。应用MTT法检测细胞增殖活性;流式细胞仪检测细胞周期分布;染色法检测细胞凋亡率。结果:与对照组比较,不同浓度依地福新处理Hela细胞24～96h后,细胞增殖显著受到抑制,并呈浓度及时间依赖性;1.0、5.0、10.0μmol.L-1浓度依地福新处理72h后,Hela细胞G0/G1期细胞数量显著增加,S期细胞数量显著降低(P<0.01);各浓度组细胞凋亡率均显著增加(P<0.01)。结论:依地福新对Hela细胞具有生长抑制作用,其机制可能与阻滞细胞周期及诱导凋亡有关。

Effect of Edelfosine on the Proliferation of Hela Cells and Its Mechanism

OBJECTIVE： To investigate the effect of edelfosine on the proliferation of Hela cells and its mechanism. METHODS： Hela cells were treated with edelfosine at doses of 0 （control）, 0.5, 1.0, 5.0, 10.0 μmol·L^-1 for 96 h. MTT assay, flow cytometry, and staining were performed to determine the cell proliferation activity, cell cycle, and apoptotic rate. RESULTS： As compared with control, the cell proliferation activity of Hela cells was inhibited in a dose and time dependent manner after being treated by edelfosine for 24--96 h. After being treated by edelfosine （1.0, 5.0, 10.0 μmol·L^-1） for 72 h, the number of Hela cells significantly increased in G0/G1 phase but decreased in S phase （P 〈 0.01）; and the apoptotic rate of Hela cells in different concentrations of edelfosine increased significantly （P 〈 0.01）. CONCLUSION： Edelfosine could inhibit the growth of Hela cells by blocking cell cycle and inducing apoptosis.