| BCG PPD对哮喘小鼠的CD4+ CD25+调节性T细胞及肺部病理的影响 |
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| 引用本文： | 陶松雪,潘家华,楼皖玲,刘辉,何金根. BCG PPD对哮喘小鼠的CD4+ CD25+调节性T细胞及肺部病理的影响[J]. 安徽医学, 2009, 30(3): 215-218. DOI: 10.3969/j.issn.1000-0399.2009.03.001 |
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| 作者姓名： | 陶松雪 潘家华 楼皖玲 刘辉 何金根 |
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| 作者单位： | 安徽省立医院儿科,合肥,230001;安徽省立医院儿科,合肥,230001;安徽省立医院儿科,合肥,230001;安徽省立医院儿科,合肥,230001;安徽省立医院儿科,合肥,230001 |
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| 基金项目： | 安徽省教育厅资助课题 |
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| 摘 要： | 目的通过建立小鼠哮喘模型,以卡介苗(BCG)和结核菌素纯蛋白衍生物(PPD)作干预因素,从而了解哮喘小鼠CD4+ CD25+调节性T细胞和其它T细胞亚群数量的变化,以及肺部的病理改变。方法将36只6～8周龄的BALB/C小鼠随机分成A、B、C、D4组,A组为BCG+BCG干预组,B组为BCG+PPD干预组,C组为哮喘模型组,每组各10只,D组6只为正常对照组。应用流式细胞仪分别检测各组的CD4+ CD25+调节性T细胞和T细胞亚群,同时取各组的肺组织PAS染色鉴定黏液分泌。结果A、B、C三组的CD3+、CD4+、CD8+ T细胞的百分比以及CD4+/CD8+的比值均比D组(正常对照组)低(P<0.05),而A、B两组的CD3+T细胞百分比较C组增高(P<0.05)。CD4+ CD25+ Treg占CD4+T细胞的百分比,其它三组低于D组(P<0.05),A、B两组较C组升高(P<0.05),而A、B两组之间的差异无统计学意义(P>0.05)。肺组织病理提示D组未见到黏液分泌;C组支气管壁可见杯状细胞增生、变大,并有大量黏液分泌;A、B两组与C组比较,杯状细胞减少,黏液分泌减少。B组气道的炎症反应轻于A组。结论...
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| 关 键 词： | 卡介苗 结核菌素纯蛋白衍生物 支气管哮喘 T细胞亚群 CD4+CD25+Treg |
The effect of bacillus Calmette-guerin vaccination and PPD on CD4~+ CD25~+ regulatory T cells and the lung pathology in a murine model of asthma |
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| Tao Songxue,Pan Jiahua,Lou Wanling,et al. The effect of bacillus Calmette-guerin vaccination and PPD on CD4~+ CD25~+ regulatory T cells and the lung pathology in a murine model of asthma[J]. Anhui Medical Journal, 2009, 30(3): 215-218. DOI: 10.3969/j.issn.1000-0399.2009.03.001 |
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| Authors: | Tao Songxue Pan Jiahua Lou Wanling et al |
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| Affiliation: | Tao Songxue,Pan Jiahua,Lou Wanling,et alDepartment of Pediatrics,Anhui Provinical Hosital,Hefei 230001 |
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| Abstract: | Objective This study examined the effect of BCG vaccination and PPD on CD4+ CD25+ regulatory T cells in asthmatic mice and its preventive effect on airway inflammation.Methods 36 BALB/C mice were divided into four (4) groups (group A, B, C and D) randomly. There were 10 mice in group A, B and C respectively,and there were six (6) mice in group D. The mice of group A, B, and C were sensitized and challenged with ovalbumin (OVA) to establish asthmatic models. Mice in Group A were injected with BCG twice, that... |
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| Keywords: | CD4 CD25 Treg |
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