芫花醋炙品中黄酮类成分的分离与鉴定

目的更好地开发利用芫花(Daphne genkwa Sieb.et Zucc)药用植物资源。方法用硅胶、羟丙基葡聚糖凝胶LH-20柱色谱、HPLC分离纯化;根据理化性质和NMR谱数据鉴定其结构。结果从醋炙芫花的乙醇提取物中分离得到9个黄酮类化合物,分别鉴定为芫花素(genkwanin,1)、3′-羟基芫花素(3′-hydroxy-genkwanin,2)、芹菜素(apigenin,3)、洋芹素-4′,7-二甲醚(4′,7-dimethylapige-nin,4)、椴苷(kaempferol-3-β-D-(6-O-trans-p-coumaroyl)glucopyranoside,5)、4′,5-二羟基-3′,7-二甲氧基黄酮(4′,5-dihydroxy-3′,7-dimethoxyflavone,6)、4′,5,7-三甲氧基黄酮(4′,5,7-trimethoxyfla-vone,7)、山奈酚-3-O-β-D-葡萄糖苷(kaempferol-3-O-β-D-glucoside,8)、3′,4′,5,7-四甲氧基黄酮(3′,4′,5,7-tetramethoxyflavone,9)。结论化合物1~9均为首次从芫花醋炙品中分离得到,化合物7、8为首次从瑞香属植物中分离得到,化合物9为首次从芫花中分离得到。

Isolation and identification of flavanoids from buds of Daphne genkwa Sieb.et Zucc.processed by rice vinegar

Objective To study the chemical constituents of the buds of Daphne genkwa sieb.et Zucc. processed by rice vinegar. Methods The constituents were isolated by chromatography methods such as silica gel, Sephadex LH-20 , ODS column and HPLC . Their chemical structures were elucidated on the basis of physico-chemical properties and spectral data. Results Nine flavanoids were obtained and their structures were identified as genkwanin(1), 3’-hydroxy-genkwanin (2), apigenin(3), 4´,7-dimethylapigenin(4), kaempferol-3-β-D-(6-O-trans-p-coumaroyl)glucopyranoside(5), 3´,7-dimethoxy-4´,5-dihydroxyflav-one(6), 4´,5,7-trimethoxyflavone(7), kaempferol-3-O-β-D-glucoside(8), 3´,4´,5,7-tetramethoxyflavone(9). Conclusions Compounds 1-9 are isolated from buds of Daphne genkwa Sieb.et Zucc. processed by rice vinegar for the first time, while compounds 7 and 8 are firstly isolated from buds of Daphne genus, and compound 9 is isolated from buds of Daphne genkwa Sieb.et Zucc.for the first time.