乙型肝炎病毒表面抗原弱反应性标本的检测及临床意义

目的探讨乙型肝炎病毒表面抗原(HBsAg)弱反应性标本的临床检测对策及其临床意义。方法采用酶联免疫吸附试验(ELISA)试剂Ⅰ、Ⅱ和化学发光法分别检测155例HBsAg初检为弱反应性的标本。结果试剂Ⅰ与化学发光法的阳性符合率为47.1%,阴性符合率为5.2%;试剂Ⅱ与化学发光法的阳性符合率为13.5%,阴性符合率为39.3%;试剂Ⅰ与试剂Ⅱ的阳性符合率为25.2%,阴性符合率为7.1%。结论对于ELISA法检测HBsAg为弱反应性的标本,应分析原因并采用多家试剂及更灵敏的方法进一步复查以确认,最好是进行确认实验。

The significance in the detection of clinical HBsAg weak reactive samples

Objective To investigate the detection countermeasures and singinficance of clinical HBsAg weak reactive samples. Methods 155 HBsAg weak reactive samples were detected hy ELISA （reagent Ⅰand Ⅱ）and chemiluminescence（CLIA）. Results The positive coincidence tale of reagent Ⅰand CLIA was 47. 1%, while the negative coincidence rate was 5.2 % ; the positive coincidence rate of reagent Ⅱ and CLIA was 13.5%, while the negative coincidence rate was 39.3% ; the positive coincidence rate of reagent Ⅰ and reagent Ⅱ was 25.2% ,while the negative coincidence rate was 7.10%. Conclusion It is important to set up a confirmative procedures to detect the clinical HBsAg weak reactive samples by ELISA in clinic.