Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers |
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Authors: | Hawkes Joanna S Bryan Dani-Louise Gibson Robert A |
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Institution: | (1) Australia;(2) Department of Paediatrics and Child Health, Flinders University, Bedford Park, South, Australia |
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Abstract: | Samples of milk (n = 80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1 , interleukin-6, and tumor necrosis factor- in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1 , interleukin-6, and tumor necrosis factor- than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40–50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1 , interleukin-6, and tumor necrosis factor- in the mammary gland and have the capacity to respond to further stimulation after leaving the breast. |
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Keywords: | Human milk human milk cells peripheral blood mononuclear cells interleukin-1 " target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0"> interleukin-6 tumor necrosis factor- " target="_blank">gif" alt="agr" align="BASELINE" BORDER="0"> |
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