妊娠高血压综合征患者内皮细胞损伤的研究

目的 探讨妊娠高血压综合征（妊高征）患者血管内皮细胞损伤的原因和机理。方法 将10例重度妊高征患者（妊高征组）和10例非妊高征孕妇（非妊高征组）的血清分别和人脐静脉内皮细胞共同培养48h,同时加入或不加人正常人中性粒细胞,观察内皮细胞凋亡的情况和细胞周期的变化。结果 不加入中粒细胞时,两组均无明显的内皮细胞凋亡发生;加入中性粒细胞后,两组都有凋亡细胞出现,妊高征组细胞凋亡的比例明显高于非妊高征组;加入或不加入中性粒细胞,两组细胞周期都表现为G2/M期比例明显降低,各组间细胞周期变化无明显差异。结论 妊高征患者的血清可能是通过活化性粒细胞,诱导内皮细胞凋亡,间接导致内皮细胞的损伤。

Study on endothelial cell injury in women with pregnancy induced hypertension

Objective To investigate the mechanisms of endothelial cell injury in women with pregnancy induced hypertension (PIH). Methods Human umbilical endothelial cells were cultured in the serum from 10 women with severe PIH and 10 normal pregnant women, and co-cultured with neutrophils or not for 48hrs. The morphology and cell cycles were studied. Results Without neutrophils, no apoptotic cells were observed, while neutrophils were added, apoptotic cells were obviously detected. The percentage of apoptotic cells in PIH was more than that in non-PIH. The percentage of cells in G 2/M phase decreased in all the groups, but there were no significant differences among them. Conclusions Endothelial cells were injured indirectly in PIH patients. Activated neutrophils induced apoptosis might be one of the answers.