吸烟和年龄对电子垃圾拆解工人尿液中8-羟基脱氧鸟苷的影响

目的 研究吸烟和年龄对电子垃圾拆解工人尿液中8-羟基脱氧鸟苷(8-hydroxy-2'-deoxyguanosine,8-OHdG)的影响.方法 采用固相萃取-高效液相色谱电化学检测器(SPE-HPLC-ECD)方法,检测64名电子垃圾拆解男性工人上班前和下班后尿液中8-OHdG浓度.按照吸烟情况和工人的年龄分别对数据进行统计分析.结果 相对于吸烟者(42名),不吸烟者(22名)尿中8-OHdG浓度明显的增高,上班前,不吸烟组的工人尿液中的8-OHdG浓度为(8.25±4.23)μmol/mol肌酐,吸烟组工人尿液中8-OHdG浓度为(5.44±1.18)μmol/mol肌酐,两者差异无统计学意义(t=-0.81,P=0.42).而在下班后,不吸烟组的工人尿液中的8-OHdG浓度为(43.12±16.19)μmol/mol肌酐,大约是吸烟组工人尿液中8-OHdG浓度[(14.82±2.51)μmol/mol肌酐]的3倍.经过1 d暴露下班后,吸烟和不吸烟工人尿液中的8-OHdG浓度差异具有统计学意义(t=-2.33,P<0.05).按照工人的年龄分组,上班前,19岁～(6名)、20岁～(22名)、30岁～(23名)、40～49岁(11名)组工人尿中8-OHdG浓度分别为(1.86±0.66)、(3.57±0.54)、(8.12±4.10)、(11.39±3.70)μmol/mol肌酐,组间差异没有统计学意义(F=0.98,P=0.41);但经过1 d的暴露,下班后,19岁～、20岁～、30岁～、40～49岁组工人尿中8-OHdG浓度分别为(4.19±2.85)、(19.89±5.26)、(28.89±14.61)、(34.94±12.50)μmol/mol肌酐,组间差异具有统计学意义(F=4.81,P=0.03).结论 吸烟对拆解工人尿中8-OHdG浓度有一定的抑制作用,尿中8-OHdG浓度随年龄增高而增加.

Smoking status or age impact on urinary 8-hydroxy-2'-deoxyguanosine in electrical and electronic equipment waste dismantling workers

Objective To investigate the smoking or age impact on occupational workers in electrical and electronic equipment waste (e-waste) dismantling procedure, using 8-Hydroxy-2'-deoxyguanosine (8-OHdG) in the urine as a biomarker for oxidative damage to DNA. Methods The pre-workshift and post-workshift urinary samples of 64 male workers in e-waste dismantling procedure were detected by solid-phase extraction-high performance liquid chromatography-electrochemical detector (SPE-HPLC-ECD). The data were statistically analyzed by two factors,age or smoking status. Results The 8-OHdG levels in non-smokers' urines (n= 42) were higher than those in smokers' urines (n=22). The levels in pre-workshift urines were detected at (8. 25±4.23)μmol/mol creatinine in non-smokers, while the values were (5.44±1.18 ) μnol/mol in smokers. And, the levels in post-workshift were detected at (43.12 ±16.19)μmoL/mol creatinine in non-smokers,while the values were(14.82±2.51 )μmol/mol in smokers. The 8-OHdG levels in pre-workshift urines were not different between non-smokers and smokers (t =-0.81 ,P=0.42) ,however after 1 day exposure,urinary 8-OHdG levels were significantly increased in non-smokers than those in smokers (t =-2.33, P <0.05). On the other hand, the subjects were divided into five groups according to their age. The 8-OHdG levels in pre-workshift urines were (1.86±0.66 ), (3.57±0.54), (8.12±4.10 ), (11.39±3.70 )μmoL/mol creatinine in < 20 years group (n=6), 20 -years group (n=22),30- years group (n=23),40-49 years group (n=11) respectively. No effect of age was found on the pre-workshift urinary 8-OHdG levels (F =0. 98 ,t =0.41). However,it was found that the post-workshift urinary 8-OHdG levels increased along with the e-waste workers' age (F=4.81, P = 0.03) ,and they were (4.19±2.85 ), (19. 89±5.26 ), (28. 89±14.61 ), (34.94±12.50) μnol/mol creatinine in <20 years group, 20- years group, 30 - years group, 40 -49 years group respectively. Conclusion The urinary 8-OHdG levels in the e-waste dismantling workers might be inhibited by smoking status. The post-workshift urinary 8-OHdG levels increased along with the e-waste workers' age.