| CCK-8法评价口腔粘结材料的体外细胞毒性研究 |
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| 引用本文： | 邹颖,王文梅,朱雅男,杨卫东. CCK-8法评价口腔粘结材料的体外细胞毒性研究[J]. 口腔医学研究, 2011, 27(8): 673-675 |
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| 作者姓名： | 邹颖 王文梅 朱雅男 杨卫东 |
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| 作者单位： | 南京大学口腔医学院·南京市口腔医院黏膜科 江苏南京210008 |
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| 摘 要： | 目的：CCK-8法评价口腔粘结材料的体外细胞毒性研究。方法：应用CCK-8比色法比较体外4种粘结材料（普通型玻璃离子、加强型玻璃离子、SuperBond C＆B及SE BOND）对人牙周膜成纤维细胞（human periodontalligament cell,HPDLC）的细胞毒性,初步探讨4种粘结材料的生物学性能。结果：4种粘结材料对HPDLC的生物相容性影响结果相似,其中普通型玻璃离子及SuperBond C＆B无细胞毒性,加强型玻璃离子具极轻微细胞毒性,SE BOND具轻微细胞毒性。结论：4种粘结材料均满足口腔材料临床应用的生物相容性要求。
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| 关 键 词： | CCK-8法 粘结材料 人牙周膜成纤维细胞 细胞毒性 |
In Vitro Evaluation the Cytotoxicity of Dental Bonding Agents Through CCK-8 Assay |
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| Affiliation: | ZHOU Ying,WANG Wen-mei,ZHU Ya-nan,et al.School of Stomatology,Nanjing University,Nanjing 210008 |
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| Abstract: | Objective：To evaluate the agtotoxicity of bonding agents in vitro using CCK-8.Methods：We use the human periodontal ligament fibroblasts in vitro to evaulate the biocompatibilities of 4 kinds of bonding agents by applying CCK-8 assay,in order to select the best bonding materials,and to find bioactive materials which are in favor of periodontal functional healing to guide the clinical.Results：The result shows that the impacts of the 4 materials on the biocompatibility of HPDLCs were similar,in which the conventional glass ionomer and SuperBond CB had no cytotoxicity,resin enforced glass ionomer had minimal toxicity and SE BOND had a slight cytotoxicity.Conclusion：4 kinds of bonding materials all meet the requirements of biocompatibility for clinical application. |
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| Keywords: | CCK-8 assay Bonding agents HPDLCs Cytotoxicity |
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