富血小板血浆对珊瑚支架上骨髓基质细胞异位成骨的影响

目的：评价富血小板血浆（platelet rich plasma,PRP）对珊瑚支架上骨髓基质细胞（marrow stromal cells,MSCs）异位成骨的影响。方法：将MSCs悬液与同一供体来源的PRP混合后滴加到珊瑚圆片上,形成珊瑚/MSCs/PRP复合物。以同样数量MSCs或PRP制备珊瑚/MSCs和珊瑚/PRP复合物。将3种复合物植入同一裸鼠的背部皮下。术后4周和8周取材,通过组织学观察和组织形态测量分析评价其异位成骨情况。结果：植入后4周或8周,珊瑚/MSCs/PRP组珊瑚表面及其孔洞内有大量的软骨或骨质形成;珊瑚/MSCs组珊瑚表面及其孔洞内也有软骨或骨质形成,并有纤维结缔组织长入;珊瑚/MSCs/PRP组形成的软骨或骨质的量明显多于珊瑚/MSCs组（P〈0.01）;珊瑚/PRP组珊瑚周围及其孔洞内有大量纤维组织,未见骨或软骨形成。结论：PRP促进了珊瑚支架上MSCs的异位成骨。

Influence of Platelet-rich Plasma on Ectopic Bone Formation of Bone Marrow Stromai Cells in Porous Coral

Objective：To evaluate the effect of platelet rich plasma（PRP） on ectopic bone formation of marrow stromal cells（MSCs） in porous coral.Methods：Natural coral disks with diameter of 8 mm and thickness of 2 mm were used in this study.A suspension of 5×106 MSCs in 50μl medium were dissolved in 50μl of PRP.The mixture of MSCs and PRP was spread on a coral disc.The constructs were implanted into the dorsal subcutaneous area of athymic mice.Coral scaffolds seeded with MSCs alone or added with PRP alone acted as control.The ectopic bone formation was investigated by histological observation and histomorphometric analyses 4 and 8 weeks after operation.Results：New bone and /or cartilage formation could be observed in specimens from both coral/MSCs/PRP group and coral/MSCs group in ectopic sites,and osteogenesis followed the pattern of endochondral bone formation.Histomorphometric analyses showed enhanced cartilage and/or bone formation in coral/MSCs/PRP group 4 and 8 weeks after implantation,compared with the coral/MSCs group.In contrast,no bone or cartilage formation could be observed in coral/PRP group.Conclusion：Combination of MSCs with PRP in porous coral could improve increased ectopic bone formation.