尼古丁对人牙周膜成纤维细胞中Fas/Apo-1(CD95)表达的影响 |
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引用本文: | 高丽,刘琪,冯萍,葛颂,何权敏.尼古丁对人牙周膜成纤维细胞中Fas/Apo-1(CD95)表达的影响[J].口腔医学研究,2011,27(10):877-880. |
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作者姓名: | 高丽 刘琪 冯萍 葛颂 何权敏 |
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作者单位: | 1. 遵义医学院口腔学院口腔内科 贵州遵义563003 2. 贵阳市口腔医院 |
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基金项目: | 贵州省遵义医学院硕士启动基金(编号:f-152) |
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摘 要: | 目的:研究尼古丁(nicotine)对体外培养的人牙周膜成纤维细胞(periodontal ligament fibroblasts,PDLFs)中Fas/Apo-1(CD95)表达的影响。方法:采用组织块培养法培养原代人牙周膜成纤维细胞并传代取处于对数生长期的第6代细胞用于实验,采用浓度为2mg/L的尼古丁处理第6代牙周膜成纤维细胞72h,通过免疫组织化学染色法检测尼古丁对人牙周膜成纤维细胞中Fas表达的情况。结果:证实尼古丁作用于人牙周膜成纤维细胞后,胞膜中Fas/Apo-1(CD95)抗原呈阳性表达。结论:提示尼古丁可通过Fas系统这一途径导致人牙周膜成纤维细胞的凋亡,从而加重牙周组织破坏的程度和影响牙周组织的再生。
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关 键 词: | 尼古丁 人牙周膜成纤维细胞 Fas/Apo-1(CD95)表达 细胞培养 |
Effect of Nicotine on Expression of Fas/Apo-1(CD95)in Periodontal Ligament Fibroblasts in Vitro |
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Institution: | GAO Li,LIU Qi,FENG Ping,et al.Hospital of Stomatology,Zunyi Medical College,Zunyi,563003 |
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Abstract: | Objective: To observe the expression of Fas-antigen(Fas/Apo-1(CD95)) of nicotine in periodontal ligament fibroblasts in vitro.Methods: Human primary periodontal ligament fibroblasts were cultured in vitro,and the sixth passage cells were used in the experiments.PDLFs were cultured in the presence of nicotine(2μg/ml) for 72h.The expression of Fas/Apo-1(CD95) was detected by immunohistochemistry in periodontal ligament fibroblasts in vitro.Results: The expression of Fas/Apo-1(CD95) antigen was positive in PDLFs cultured in the presence of nicotine with immunohistochemical method.Conclusion: Nicotine can induce apoptosis in periodontal ligament fibroblasts in vitro by Fas system. |
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Keywords: | Nicotine Periodontal ligament fibroblasts(PDLFs) Fas/Apo-1(CD95) Cell culture |
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