淫羊藿苷对人牙周膜细胞增殖及骨向分化的影响

目的：探讨淫羊藿苷对人牙周膜细胞增殖及骨向分化的影响,为淫羊藿苷在治疗牙周病方面的应用提供一定依据。方法：体外培养人牙周膜细胞,矿化诱导条件下将第3代细胞与10～0.001mg/L,6个浓度的淫羊藿苷作用,通过噻唑蓝（MTT）比色法、ALP活性测定及BSP表达水平,反应其对人牙周膜细胞增殖及分化的影响。结果：作用24h,各药物浓度均能促进人牙周膜细胞增殖（P〈0.05）。作用48h,1～0.001mg/L组可促进人牙周膜细胞增殖（P〈0.05）。作用72h,0.1～0.001mg/L组可促进人牙周膜细胞增殖（P〈0.05）,10mg/L组抑制人牙周膜增殖（P〈0.05）;作用72h,1～0.001mg/L组可促进人牙周膜碱性磷酸酶（ALP）活性（P〈0.05）;作用72h,0.1～0.001mg/L组的BSP表达水平较对照组显著增加（P〈0.05）。结论：淫羊藿苷在一定浓度范围内可促进人牙周膜细胞增殖及骨向分化。

Effects of Icariin on the Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Cells

Objective： To investigate the effects of icariin on the proliferation and osteogenic differentiation of human periodontal ligament cells in order to provide a basis for the application of icariin in periodontal disease treatment.Methods： Human periodontal ligament cells were cultured in vitro.Under the conditions of mineralization induce,the third passage of human periodontal ligament cells were treated with 6 different concentrations（10-0.001μg/ml） of icariin.MTT colorimetric assay,ALP and BSP were performed to assess the effects of icariin on the proliferation and osteogenic differentiation.Results： All concentrations of icariin promoted proliferation of human periodontal ligament cells for 24 hours（P0.05）.1-0.001μg/ml icariin promoted proliferation of human periodontal ligament cells for 48 hours（P0.05）.0.1-0.001μg/ml icariin promoted proliferation of human periodontal ligament cells for 72 hours（P0.05）,while 10μg/ml icariin inhibited proliferation of human periodontal ligament cells（P0.05）.1-0.001μg/ml icariin improved ALP activity expression for 72 hours（P0.05）.Compared with the control group,BSP expression of 0.1-0.001μg/ml cariin were increased significantly for 72 hours（P0.05）.Conclusion： Using appropriate icariin could promote proliferation and osteogenic differentiation of human periodontal ligament cells.