替罗非班对大鼠心肌缺血再灌注后左心室重塑及心功能的影响

目的观察替罗非班对大鼠心肌缺血再灌注后左心室重塑及心功能的影响。方法雄性Wistar大鼠36只,随机分为假手术组、模型对照组和替罗非班组各12只。采用冠状动脉结扎法建立大鼠心肌缺血再灌注模型,假手术组只穿线不结扎。术后28d存活大鼠测定血流动力学及心功能。用苦味酸天狼猩红染色,测定左心室梗死区(假手术组为左心室)游离壁厚度(LVMT)、室间隔厚度(SPT)、窜间隔厚度,左心室游离壁厚度(SPT/LVMT)、室间隔的心肌横断面积(MAAS)、梗死区(假手术组为左心室游离壁)及非梗死区的胶原容积分数(CVF),并进行定量分析。结果血流动力学分析显示:模型对照组和替罗非班组大鼠的收缩压(SBP)、舒张压(DBP)、左心窜收缩压(LVSP)、左心室内最大上升及下降速率(±dp/dt_(max))低于假手术组,左心室舒张末压(LVEDP)高于假手术组(P<0.01,P<0.05);替罗非班组大鼠的±dp/dt_(max)高于模型对照组(P<0.05),LVEDP低于模型对照组(P<0.05),SBP、DBP、LVSP较模型对照组有升高的趋势,差异无统计学意义(P>0.05);缺血再灌注后28d,模型对照组和替罗非班组大鼠的LVMT低于假手术组(P<0.01),SFT/LVMT、MAAS及梗死区CVF高于假手术组(P<0.01),SFF及非梗死区CVF 3组间差异无统计学意义(P>0.05);替罗非班组大鼠LVMT高于模型对照组(P<0.05);SPT/LVMT、MAAS、梗死区CVF低于模型对照组(P<0.01)。结论替罗非班可抑制大鼠心肌缺血再灌注后的左心室重塑,改善心功能。

Effects of tirofiban on ventricular remodeling and cardiac functi on after myocardial ischemia/reperfusion in rats

Objective To investigate effects of tirofiban on ventricular remodeling and cardiac function after myocardial ischemia/reperfusion in rats.Methods 36 male Wistar rats were randomized into three groups, sham operation group, model control group and tirofiban treatment group（ 12 rats in each group）. The animal models of myocardial ischemia/reperfusion were established. The rats in control group and tirofiban group were subjected ischemia for 90 minutes by ligation of coronary artery, after thoracotomy and sequent persistent repeffusion. While the rats in sham operation group didn＇t underwent occlusion of coronary artery. On the 28th day after operation, hemodynamic parameters and cardiac function in all survival rats were evaluated. The rats were killed. Picrosirius red staining plus light microscopy was used to analyze quantitatively the thickness of left ventricular free wall （LVWT） in infracted region（ left ventricular free wall for sham group）, septum（SPT） and ratio of SPT/LVWT, myocardial across area in septum（MAAS） and collagen volume fraction （CVF） in non-infarcted and infracted region（ left ventricular free wall for sham group）. Results The hemodynamics analysis showed SBP, DBP, LVSP, ± dp/dtmax in model control group and tirefiban treatment group were significantly lower than those of sham operation group, however, LVEDP was obviously higher than that of sham operation group（ P 〈 0.01 or P 〈 0.05）.The ± dp/dtmax in tirofiban treatment group was significantly higher than that of model control group（ P 〈 0.05） ,but LVEDP was lower than that of model control group（ P 〈 0.05）. Although SBP, DBP, LVSP in tirofiban treatment group were higher than those of model control group, there were no significant differences between two groups（ P 〉 0.05）. After 28-day ischemia/reperfusion, LVMT in in model control group and tirofiban treatment group was significantly lower than that of sham operation group（ P 〈 0.01 ）, however, SPT/LVMT, MAAS, CVF in infracted