黄连素对子宫肌瘤大鼠模型血液黏度、子宫系数、平滑肌厚度和炎性细胞浸润的影响

目的分析黄连素对子宫肌瘤大鼠模型血液黏度、子宫系数、平滑肌厚度和炎性细胞浸润的影响。方法取32只成年SPF级SD大鼠,随机分为对照组、模型组、低剂量黄连素组、高剂量黄连素组,每组各8只。除对照组外,其余三组均给予苯甲酸雌二醇注射液+黄体酮注射液肌内注射建立子宫肌瘤模型。对照组和模型组均给予等量生理盐水,低剂量黄连素组采用黄连素10 mg/kg进行灌胃,高剂量黄连素组采用黄连素20 mg/kg进行灌胃。比较各组大鼠血液黏度、血清肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、α干扰素(INF-α)、子宫重量、子宫系数和平滑肌厚度,经病理学染色观察各组大鼠子宫形态学状况。结果模型组大鼠红细胞聚集指数、血浆黏度、全血黏度均明显高于对照组,低剂量黄连素组、高剂量黄连素组大鼠红细胞聚集指数、血浆黏度、全血黏度均明显低于模型组(P<0.05)。相比对照组,模型组大鼠血清TNF-α和IL-6水平显著增高,INF-α水平显著降低(P<0.01);低剂量黄连素组和高剂量黄连素组血清TNF-α和IL-6水平均明显低于模型组,INF-α水平均明显高于模型组(P<0.01)。模型组大鼠子宫重量、子宫系数和平滑肌厚度较对照组均明显增加,低剂量黄连素组和高剂量黄连素组大鼠子宫重量、子宫系数和平滑肌厚度较模型组均明显下降(P<0.05)。相比对照组,模型组、低剂量黄连素组和高剂量黄连素组出现不同程度的炎性细胞浸润,其中高剂量黄连素组的炎症程度较轻。结论黄连素对子宫肌瘤模型大鼠具有良好的治疗效果,能降低其血液黏度、子宫系数、平滑肌厚度和炎性细胞浸润程度,作用机制可能在于黄连素具有改善大鼠体内炎性因子水平的效果。

Effects of berberine on blood viscosity,uterine coefficient,smooth muscle thickness and inflammatory cell infiltration in rat model of hysteromyoma

Objective To analyze the effect of berberine on blood viscosity,uterine coefficient,smooth muscle thickness and inflammatory cell infiltration in the rat model of hysteromyoma.Methods 32 adult SPF SD rats were randomly divided into control group,model group,low dose berberine group and high dose berberine group.In addition to the control group,the other three groups were given estradiol benzoate injection+ progesterone injection intramuscular injection to establish uterine fibroids model.The control group and the model group were given the same amount of normal saline,the low-dose berberine group was given 10 mg/kg berberine,the high-dose berberine group was given 20 mg/kg berberine.The blood viscosity,serum tumor necrosis factor α(TNF-α),interleukin 6(IL-6),α interferon(INF-α),uterus weight,uterus coefficient and smooth muscle thickness of each group were compared,and the uterus morphology of each group was observed by pathological staining.Results The red blood cell aggregation index,plasma viscosity and whole blood viscosity of model group were significantly increased in comparison of control group,while those of low-dose berberine group and high-dose berberine group were significantly decreased in comparison of model group(P<0.05).Compared with the control group,the serum TNF-α and IL-6 level of the model group was significantly increased and the INF-α level was significantly decreased(P<0.01).The serum TNF-α and IL-6 level of the low-dose berberine group and the high-dose berberine group was significantly decreased and the INF-α level was significantly increased in comparison of model group(P<0.01).The uterus weight,uterus coefficient and smooth muscle thickness in the model group were significantly increased in comparison of the control group,while the uterus weight,uterus coefficient and smooth muscle thickness in the low-dose berberine group and the high-dose berberine group were significantly decreased in comparison of the model group(P<0.05).Compared with the control group,model group,low-dose berberine group and high-dose berberine group had different degrees of inflammatory cell infiltration,and the high-dose berberine group had less inflammatory degree.Conclusion Berberine has a good therapeutic effect on the rat model of hysteromyoma.The mechanism may be that berberine can improve the level of inflammatory factors in the body of the rat model of hysteromyoma.