| 白喉棒状杆菌在实验教学中的染色方法改良 |
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| 作者姓名： | 陈剑煌 周红 黄锦桃 李美玉 |
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| 作者单位： | 中山大学中山医学院病原生物学实验室 |
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| 基金项目： | 中山大学本科教学质量工程类项目(中大教务【2018】294号)。 |
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| 摘 要： | 目的探索实验教学中对白喉棒状杆菌的染色方法的改良。方法将复苏的白喉棒状杆菌接种于鸡蛋培养基上培养24 h,挑取菌体涂片,俾斯麦褐液染色1 min,后以奈瑟氏美蓝液染色1.5 min,制作得染色玻片。结果在油镜下可见清晰的蓝色菌体和明显的深蓝色异染颗粒。在较强及较弱的光线条件下菌体轮廓均可辨认。结论改良的染色方法可改进实验教学效果,其在临床上的适用性有待进一步研究。
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| 关 键 词： | 白喉棒状杆菌 染色 改良 实验教学 |
Staining improvement of Corynebacterium diphtheria in experimental teaching |
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| Authors: | Jianhuang CHEN Hong ZHOU Jintao HUANG Meiyu LI |
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| Institution: | (Laboratory of Pathogenic Biology,Zhongshan Medical College,Zhongshan University,Guangzhou 510008,China) |
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| Abstract: | Objective To reaserch the improvement of staining method of Corynebacterium diphtheriain experimental teaching.Method Cultured the resuscitated Corynebacterium diphtheriaon Egg-medium for 24 hours.Picked bacteria to smear slide,which was stained with Bismarck brown liquid for 1 minute,then with Methylene blue liquid for 1.5 min.Result Clear blue thallus and distinct dark blue metachromatic granule were seen in the oil objective len.Conclusion This improved staining method provided a better experimental teaching effect.The clinical applicability of this method remains to be farther studied. |
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| Keywords: | Corynebacterium diphtheria Staining Improve Experimental teaching |
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