| 北京市男男性接触HIV-1感染者膜蛋白基因序列测定及亚型分析 |
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| 引用本文： | 张洪海,孙玉,柳雅立,吴亚松,代丽丽,吴昊,陈德喜.北京市男男性接触HIV-1感染者膜蛋白基因序列测定及亚型分析[J].中华实验和临床病毒学杂志,2009,23(4). |
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| 作者姓名： | 张洪海 孙玉 柳雅立 吴亚松 代丽丽 吴昊 陈德喜 |
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| 作者单位： | 首都医科大学附属北京佑安医院,100069 |
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| 基金项目： | 国家自然科学基金面上项目，北京市科委科技计划重大项目 |
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| 摘 要： | 目的 了解北京市男男性接触人群(MSM)中HIV-1的最新流行趋势及膜蛋白V3环序列特征.方法 巢式聚合酶链式反应(n-PER)扩增2007年提取的北京市男男性接触HIV感染者基因组DNA样品,对膜蛋白基因C2-V3区测序,进行病毒亚型及V3环序列特点分析.结果 11例样本中,4例是欧美B亚型,5例是AE重组亚型,1例是BC重组亚型,1例是01B重组亚型.V3环顶端四肽以GPGQ和GPGR为主.结论 北京市男男性接触HIV-1感染者中重组亚型呈蔓延流行趋势.
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| 关 键 词： | 性传播性疾病 病毒性 基因 |
Env gene sequencing and HIV-1 subtyping of infected MSM from Beijing |
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| ZHANG Hong-hai,SUN Yu,LIU Ya-li,WU Ya-song,DAI Li-li,WU Hao,CHEN De-xi.Env gene sequencing and HIV-1 subtyping of infected MSM from Beijing[J].Chinese Journal of Experimental and Clinical Virology,2009,23(4). |
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| Authors: | ZHANG Hong-hai SUN Yu LIU Ya-li WU Ya-song DAI Li-li WU Hao CHEN De-xi |
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| Abstract: | Objective To comprehend the latest HIV-1 epidemic tendency and the character of V3 loop in MSM population of Beijing. Methods The C2-V3 regions of the HIV envelop gene were amplified by nest-PCR and sequenced from 11 HIV-1-infected MSM in Beijing in 2007. The subtype and sequences of V3 loop was analyzed. Results There are 4 subtype B strains, 5 CRF AE, 1 CRFOTBC and 1 CRFI5-01B strains within all 11 strains. There are five types of central motifs of the 11 samples, in which GPGR and GPGQ are most common. Conclusion Recombination subtype of HIV-1 are spread extensively in MSM population of Beijing. |
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| Keywords: | HIV-1 |
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