RP-HPLC法测定保健品葛根粉中葛根素的含量

目的以RP-HPLC法测定保健品葛根粉中葛根素的含量。方法以甲醇-乙腈-水(10∶10∶80)为流动相,在依利特Hypersil ODS柱(4.6 mm×200 mm,5μm),流速为1.0 ml/min,250 nm波长下进行测定,进样量10μl。结果葛根素在20μg/ml~200μg/ml时,浓度与峰面积呈良好的线性关系(r=0.999 9),加标回收率为99.37%~99.82%,最低检出限为2μg/ml,相对标准偏差RSD为0.03%~0.15%。结论本方法操作简单准确,可用于保健品葛根粉中葛根素的含量测定。

Determination of puerarin with RP-HPLC in healthy food

Objective To determine the content of puerarin in health product Ge-Gen-Fen with RP-HPLC.Methods Chromatographic conditions included Hypersil ODS column(4.6mm×200mm,5μm) and the mobile phase consisting of a mixture of methanol-etonitrile-water(10:10:80).Detection wavelength was at 250 nm.The flow rate was 1.0 ml.min-1,and the sample dose was 10μl.Results When concentration of puerarin was 20~200μg/ml,the perfect linearity between concentration and peak area ratio was obtained(r=0.999 9),the recovery rate was 99.37%～99.82%,and the detection limit was 2μg/ml,the RSD was 0.03%～0.15%.Conclusion This method was simple and accurate,and can be used to measure the content of puerarin in health product Ge Gen Fen.