骨肉瘤高转移细胞SOSP-M_1生物学稳定性及组织学起源的鉴定

目的　检测人骨肉瘤细胞高转移亚系SOSP M1 在裸小鼠体内传代过程中的生物学稳定性并鉴定其组织学特性。　方法　利用原位移植将细胞系在 33只裸鼠体内连续传代 ,组织块培养收集各代肺转移灶的肿瘤细胞 ,观察各代肿瘤细胞致瘤率、转移率、形态结构、骨形成蛋白、波形蛋白、肌动蛋白、神经元特异性烯醇化酶的表达情况以及遗传学方面的变化。　结果　各代肿瘤细胞致瘤率均为 10 0 % ,体内增殖稳定 ,肺转移率在 80 %以上。其显微及超微结构形态、抗原表达、染色体数目及结构变化均符合人骨肉瘤的特征。　结论　该细胞亚系在裸鼠体内传代生物学特性相对稳定 ,是人骨肉瘤实验研究的良好模型。

Biological stabilities and identification of a highly metastatic subpopulation from a human osteosarcoma cell line

Objective To investigate the bio logical stabilities and the identification of a human highly metastatic osteosar coma subpopulation. Methods Tumor pieces of t he cell were inoculated into nude mice orthotopically. Lung node metastatic foci were obtained by culturing, the procedure was repeated for 3 cycles. Changes we re observed in intratibial tumor growth, pulmonary metastatic rate in vivo, morp hological characteristics, expressions of BMP, vimentin, actin, NEC and karyotyp e analyses of every passage in vitro. Results The intratibial tumor growth rates were 100%, the proliferation abilities were s table, and the pulmonary metastatic rates were higher than 80%. The expressions of BMP, vimentin, actin were positive, but the expression of NSE was negative. I ts morphological characteristics the antigen-positive substances and karyotype were similar to those observed in human osteosarcoma. Conclusio n SOSP-M 1 with very stable biological characteristics is a good model for the study of human osteosarcoma.