福斯可林和3—异丁基—1—甲基黄呤嘌对成年金黄地鼠视网膜节细胞再生的影响

探讨福斯可林(forskolin)或/和3-异丁基-1-甲基黄呤嘌(IBMX)玻璃体内注射对视网膜节细胞再生的作用。材料和方法采用逆行示踪标记术，研究外周神经缝接的对照组和外周神经缝接后，玻璃体内注射forskolin和/或IBMX的实验组视网膜节细胞再生情况。结果(1)对照组(AG)术后4、6周，每个视网膜再生的节细胞数分别为1260±137个和957±175个；(2)在上述两个时间点上各实验组每个视网膜再生的节细胞数分别为①forskolin组(AG+F)1803±648个和1068±388个；②IBMX组(AG+I)2284±1005个和1002±157个；③forskolin和IBMX联合应用组(AG+I+F)4804±375个和3161±605个；其中，AG+I+F组同AG,AG+F,AG+I组相比在这两个时间点上均存在显著性差异(P＜0.001)。结论研究结果提示联合应用forskolin和IBMX可大幅提高视网膜节细胞的再生。

EFFECTS OF FORSKOLINAND/OR IBMX ON REGENERATION OF RETINAL GANGLION CELLS IN ADULT HAMSTERS

Objective: To investigate the effects of forskolin and/or IBMX on axotomized retinal ganglion cells (RGCs) in adult hamsters. Methods: Fluorescent retrograde labeling method was used to measure the quantity of RGCs regeneratin in control and experimental animals treated with forskolin and/or IBMX 4 and 6 weeks after surgery. Results: (1) In control (AG) retinas, the mean number of regenerating RGCs were 1260 ± 137/retina and 957±75/retina at 4, 6 week after surgery,respectively, (2) The mean number of regenerating RGCs in AG + F group at 4 week and 6 week were 1803±648/retina and 1068 ± 388/retina, respectively. (3) In AG+ I group, the mean number of regenerating RGCs at 4 week and 6 week were 2284 ± 1005/retina and 1002 ± 157, respectively. (4)The mean number of regenerating RGCs in AG + I + F were 4804 + 375/retina and 3161 + 605/retina at 4 week and 6 week after surgery, respectively. They were significantly different from groups of AG, AG+ F and AG+ I(P＜0.001). Conclusion: Our results indicate that forskolin combined with IBMX has significant effects in promoting the regeneration of RGCs after axotomy.