高效液相色谱法测定人血浆中羟基红花黄色素A的浓度

目的建立高效液相色谱法测定人血浆中羟基红花黄色素A的含量。方法采用Diamonsil C18色谱柱，流动相为乙腈-0．2％磷酸溶液（20：80，v／v），流速为1mL·min^-1，检测波长为400nm，柱温：室温。结果羟基红花黄色素A在浓度0．0408～4．16μg·mL^-1。与峰面积线性关系良好，羟基红花黄色素A在血浆中3个浓度的绝对回收率平均分别为98．7％、97．9％、99．8％，批内、批间RSD均〈10％。结论本方法简单快速，准确灵敏，重现性好，适用于羟基红花黄色素的血药浓度测定。

Determination of hydroxysafflor yellow A in human plasma by HPLC

Objective To establish an HPLC to determine the level of hydroxysafflor yellow A （HYSA） in human plasma. Methods Diamonsil C18 colume was used. Aeetonitrile-2% phosphoric acid solution （20 ： 80, -v/v） was used as the mobile phase at a flow rate of 1 mL · min ^-1 with detection wave length at 400 nm. Results HYSA had good linear relationship when its concentration was between 0. 040 8-4. 16 μg · mL^-1. Absolute recoveries were 98.7%, 97.9%, and 99.8% respectively. The RSD of intra-run and inter-run was less than 10%. Conclusion The method is simple, quick and convenient for the determination of HYSA.