肺癌组织中ET-1阳性表达与血管生成关系的研究

目的：研究内皮素-1（ET-1）在肺癌组织标本中的表达及与肺癌分型、分级的关系，探讨ET-1的表达与微血管密度（MVD）的关系。方法：用免疫组织化学SABC染色方法对不同肿瘤组织进行ET-1表达的测定并行MVD计数，与良性病变组及正常肺组织对照。结果：非小细胞肺癌组织中MVD显著高表达，且与肺癌原发肿瘤大小、淋巴结转移状态和细胞分化程度有密切关系。ET-1在非小细胞肺癌中阳性表达，小细胞肺癌则为阴性表达，两者之间差异具有统计学意义（P=0．008）。非小细胞肺癌ET-1含量与肺癌原发肿瘤大小、淋巴结转移状态和细胞分化程度也有密切关系。ET-1表达阳性组中其MVD显著增高，而ET-1表达阴性的肿瘤组织中MVD仅有微弱表达，两者之间差异有统计学意义（P〈0．05）。结论：ET-1在非小细胞肺癌中高表达，且与分化程度和转移状态密切相关：ET-1可能通过肺癌血管生成参与非小细胞肺癌血管发生、发展；ET-1和MVD含量可作为判断非小细胞肺癌分化程度、治疗效果、预后的有效指标。

Relationship between Positive Expression of ET-1 and Neovascularization in Lung Cancer

Objective:To study the relationship between endothelin-1(ET-1) positive expression and the types of classification of non-small cell lung cancer,observe the connection between ET-1 and microvessel density(MVD) expression and discuss the role of ET-1 in neovascularization of lung cancer.Methods:The expressions of ET-1 and MVD were detected by immuno-histochemistry.Results:The expression of MVD was notably higher in non-small cell lung cancer.The MVD was closely related to the size of primary cancer,lymph node status and grade of cell differentiation of non small cell lung cancer.The expression of ET-1 was positive in non small cell lung cancer but negative in small cell lung cancer.The positive rate of ET-1 in non small cell lung cancer was significantly higher than that in small-cell lung cancer(P = 0.008) .The expression of ET-1 was closely related to the size of primary cancer,lymph node status and grade of cell differentiation of non small cell lung cancer.The expression of MVD was notably higher in ET-1 positive expression tissues.The expression of MVD was weak in tissue with negative expression of ET-1(P < 0.05) .Conclusion:There was ET-1 positive expression in non-small cell lung cancer.ET-1 may contribute to neovascularization of non-small cell lung cancer.The expression levels of ET-1 and MVD can be used to detect the grade of cell differentiation,the treatment efficacy and prognosis of non small cell lung cancer.