慢性肾炎治疗前后血清IL-2、IL-6、IL-10、IL-18和T淋巴细胞亚群检测意义

目的探讨慢性肾炎患者治疗前后血清IL-2、IL-6、IL-10、IL-18和T淋巴细胞亚群的变化。方法分别应用放免法、ELISA法和单克隆抗体法对30例慢性肾炎患者治疗前后进行了血清IL-2、IL-6、IL-10、IL-18和T淋巴细胞亚群水平的检测,并与35名正常健康人作比较。结果慢性肾炎患者在治疗前血清IL-2和CD4/CD8比值明显低于正常人组（P〈0.05）,而IL-6、IL-10和IL-18水平高于正常人组（P〈0.01）;经半年治疗后血清IL-2、IL-6、IL-10、IL-18和CD4/CD8与治疗前组比较差异有统计学意义（P〈0.05）。结论检测慢性肾炎患者血清IL-2、IL-6、IL-10、IL-18和T淋巴细胞亚群水平对判断病情及其预后均具有一定的临床实用价值。

Clinical significance of the Changes of Serum Levels of IL-2,IL-6,IL-10,IL-18 and T cell subset in patients with chronic nephritis

Objective To explore the changes in serum IL-2、IL-6、IL-10、IL-18 and T Lymphocyte subsets before and after treatment in patients with chronic nephritis. Methods The levels of serum IL-2、IL-6 and IL-10 was detected with RIA, IL-18 were detected with ELISA and T Lymphocyte subsets distribution type was detected with monoclonal antibody in 30 cases of chronic nephritis both before and after treatment and 35 controls. Results Before treatment, The levels of serum IL-2 and CD4 /CD8 ration were significantly lower than those in controls （P〈0.01） ,while the serum levels of IL-6、IL-10 and IL-18 were significantly higher than those in controls （ P〈0. 05）. 6 months after treatment, serum IL-2、IL-6、IL-10、IL-18 and CD4 /CD8 levels remained significantly higher or lower （P〈0.05）. Conclusion Detection of serum IL-2、IL-6、IL-10、IL-18 and CD4 /CD8 ration is valuable for the diagnosis and outcome prediction in patients with chronic nephritis.