自身免疫性溶血性贫血患者外周血CD4^＋CD25^＋调节性T细胞的检测及其临床意义

目的探讨自身免疫性溶血性贫血（AIHA）患者外周血CD4^＋CD25^＋调节性T细胞水平及临床意义。方法采用流式细胞仪对25例AIHA患者治疗前后和15例正常对照者外周血CD4^＋CD25^＋调节性T细胞占CD4^＋T细胞的比例进行了检测。结果25例AIHA患者治疗前CD4^＋CD25^＋调节性T细胞占CD4^＋细胞的比例与正常对照组比较明显降低，（4．47±2．11）％VS（9．82土2．98）％（P〈0．001），治疗后CD4^＋CD25^＋调节性T细胞的比例与治疗前比较显著升高，（7．60±2．50）％VS（4．47±2．11）％（P〈0．001），但仍低于正常人，（7．60±2．50）％VS（9．82±2．98）％（P〈0．05）。治疗后完全缓解患者CD4^＋CD25^＋调节性T细胞占CD4^-T细胞的比例与治疗无效组比较明显升高，（9．55±2．36）％VS（5．68±2．67）％（P〈0．05），无效组患者CD4^＋CD25^＋调节性T细胞占CD4^-T细胞的比例与正常时照组比较仍然降低，（5．68±2．67）％vs（9．82±2．98）％（P〈0．01）。结论AIHA患者外周血CD4^＋CD25^＋调节性T细胞的比例较正常对照明显降低，有效治疗后得到恢复，提示CD4^＋CD25^＋调节性T细胞可能参与了疾病的发生。

Expression of CD4+CD25+ regulatory T cells in patients with autoimmune hemolytic anemia

Objective To explore the change of CD4^＋ CD25^＋ regulatory T cells and its clinical significance in patients with autoimmune hemolytic anemia（AIHA）. Methods The frequencies of CD4^＋ CD25^＋regulatory T cells were detected in 25 patients with AIHA before and after treatment and 15 normal controls by multi-colour flow cytometry. Results The proportion of CD4^＋ CD25^＋; regulatory T cells in CD4^＋ T cells was higher after treatment than before in AIHA patients, （7.60 ± 2.50）% vs （4.47 ±2.11 ） % （ P〈 0. 001 ）, both of which were significantly lower than that of normal controls, respectively, （7.60±2.50）% vs （9.82±2.98）% （P 〈0.05）, （4.47±2. 11）% vs （9.82±2.98） % （ P 〈0. 001）. The proportion of CD4^＋CD25^＋ regulatory T cells in CD4^＋ T cells was significantly lower in no-remission patients than that of normal controls, （5.68± 2.67）% vs （9.82 ± 2.98）% （P 〈0.05）. Conclusion CD4^＋ CD25^＋ regulatory T ceils may be involved in pathophysiology of AIHA.