Analysis of Iranian <Emphasis Type="Italic">Potato virus S</Emphasis> isolates |
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| Authors: | Khadijeh Salari Hossein Massumi Jahangir Heydarnejad Akbar Hosseini Pour Arvind Varsani |
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| Institution: | (1) Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran;(2) Electron Microscope Unit, University of Cape Town, Cape Town, 7701, South Africa;(3) Biomolecular Interaction Centre, University of Canterbury, Christchurch, 8140, New Zealand;(4) School of Biological Science, University of Canterbury, Christchurch, 8140, New Zealand; |
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| Abstract: | Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected
between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich
enzyme-linked immunosorbent assays (DAS-ELISA) using a Potato virus S (PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and
molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced.
All 12 PVS isolates showed mosaic symptoms on Nicotiana debneyii and N. tabacum cv. Whiteburly and local lesion on Chenopodium amaranticolor, C. quinoa and C. album. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVSO isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes
for the PVSO strain and 3 genotypes for the PVSA strain. |
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