High degree of Plasmodium vivax diversity in the Peruvian Amazon demonstrated by tandem repeat polymorphism analysis |
| |
| Authors: | Kosek Margaret Yori Pablo P Gilman Robert H Calderon Maritza Zimic Mirko Chuquiyauri Raul Jeri Cesar Pinedo-Cancino Viviana Matthias Michael A Llanos-Cuentas Alejandro Vinetz Joseph M |
| |
| Institution: | Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA. mkosek@jhsph.edu |
| |
| Abstract: | Molecular tools to distinguish strains of Plasmodium vivax are important for studying the epidemiology of malaria transmission. Two sets of markers-tandem repeat (TR) polymorphisms and MSP3α-were used to study Plasmodium vivax in patients in the Peruvian Amazon region of Iquitos. Of 110 patients, 90 distinct haplotypes were distinguished using 9 TR markers. An MSP3α polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using HhaI and AluI revealed 8 and 9 profiles, respectively, and 36 profiles when analyzed in combination. Combining TR and PCR-RFLP markers, 101 distinct molecular profiles were distinguished among these 110 patients. Nine TR markers arrayed along a 100 kB stretch of a P. vivax chromosome containing the gene for circumsporozoite protein showed non-linear linkage disequilibrium (I(SA) = 0.03, P = 0.001). These findings demonstrate the potential use of TR markers for molecular epidemiology studies. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
