膜杂交技术检测HPV基因分型联合液基细胞学在宫颈癌筛查中的应用

目的采用膜杂交法对人乳头瘤病毒（HPV）进行基因分型,液基细胞学检测细胞分级,用作宫颈癌筛查手段的临床价值和意义。方法采用膜杂交法检测23个HPV基因型别（低危型：HPV6、11、42、43、45和高危型：HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83、MM4）和液基细胞学（TCT）作为宫颈癌和癌前病变的筛查。结果781例临床样本中,HPV阳性360例;占46.02%,其中单一型HPV感染中,低危型占34%、高危型占60%、二型和二型以上混合感染占6%。TCT检查≥ASCUS 184例占27%。结论本地区宫颈疾患人群中,存在较高的HPV感染率;随着TCT级别的升高,HPV感染率、特别是高危型的感染率明显升高。HPV基因分型检测是有价值的辅助诊断技术,与细胞学联合,是最佳的宫颈癌筛查的方案。

Testing of HPV genotyping by using membrane hybridization technique combining thin prep cytologic test in cervical cancer screening

Objectives： To explore the clinical significance of HPV genotyping by using Membrane hybridization technique and cell grading by Thin Prep Cytologic Test in cervical cancer screening. Methods： Twenty - three types of Human Papilloma Virus （HPV） including low risk type： HPV6, 11, 42, 43, 45 and high risk type： HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83 ＆ MM4 received membrane hybridization and Thin Prep Cytologic Test （TCT） to screen cervical cancer and precancerous lesions. Results： Among the 781 cases, 360 （46.02%） were HPV positive including single HPV infection （34% of low risk type abd 60% of high risk type） and 6% mixed HPV infection . 184 cases （27%） showed TCT ≥ASCUS. Con- clusion ： There is a high prevalence of HPV infection among the patients with cervical diseases in this region. With upgrading of TCT, detection rate of HPV infection especially high - risk type of infection has increased significantly. HPV genotyping is a valuable assistant diagnosis technique. It combines TCT, which is the best technique for cervical cancer screening.