鼻咽癌外周血单个核细胞和血浆中EB病毒DNA水平变化及与放化疗疗效的关系

目的：比较鼻咽癌患者放化疗治疗前后外周血单个核和血浆EBV DNA水平的动态变化,探讨EBV DNA水平与鼻咽癌放化疗近期疗效的关系。方法采用实时荧光定量PCR方法分别测定整群选取的2015年7—11月福建省肿瘤医院放疗科收治的75例初诊鼻咽癌患者放化疗前后外周血单个核细胞和血浆EBV DNA水平,根据疗效评价分为CR、PR、SD、PD组,各组治疗前后外周血单个核细胞和血浆EBV DNA水平分别进行比较；对配对的75例治疗前鼻咽癌患者外周血单个核细胞和血浆EBV DNA水平进行检出率和相关性分析。结果 CR、PR组外周血单个核细胞和血浆EBV DNA治疗前后下降明显,差异有统计学意义(P<0.05)；SD、PD组外周血单个核细胞和血浆EBV DNA治疗前后差异无统计学意义(P>0.05)。外周血单个核细胞和血浆EBV DNA总体检出率为78.7%、53.3%,差异有统计学意义(P<0.05)；两种类型标本EBV DNA结果呈正相关(r=0.481,P=0.00)。结论外周血单个核细胞和血浆EBV DNA检测可作为评价鼻咽癌放化疗近期疗效的指标。

The Dynamic Change of Peripheral Blood Mononuclear Cell and Plasma Epstein-Barr Virus DNA in Patients with Nasopharyngeal Carcinoma and Correlation with the Radiotherapy and Chemotherapy Curative Effect

Objective To compare the levels of peripheral blood mononuclear cell and plasma Epstein-barr virus DNA in patients with nasopharyngeal carcinoma (NPC) before and after radiotherapy and chemotherapy, and to discuss the correla-tion between the levels of EBV DNA and the short-term curative effect of the radiochemotherapy. Methods Real-time fluo-rescent quantitative PCR method were determined in 2015 July to November in fujian province tumor hospital radiotherapy was treated 75 cases of nasopharyngeal carcinoma patients before and after the radiation and chemotherapy peripheral blood mononuclear cells and plasma EBV DNA level.Compare the concentrations before and after therapy in CR、PR、SD、PD groups. The relationship was analyzed between plasma and PBMCs EBV DNA. Results CR, PR of peripheral blood mononuclear cells and plasma EBV DNA before and after treatment decreased significantly, the difference was statistically significant (P<0.05);SD, PD group of peripheral blood mononuclear cells and plasma EBV DNA before and after treatment there was no statistically significant difference (P>0.05). Peripheral blood mononuclear cells and plasma EBV DNA overall detection rate of 78.7%, 53.3%, obvious difference (P< 0.05); Two types of specimens of EBV DNA results were positively correlated (r=0.481,P=0.481) Conclusion Plasma and PBMCs EBV DNA could be used to access the short-term curative effect of NPC with radiotherapy and chemotherapy.